Detailed information of TA system TA192829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1022593..1023231
• Replicon: chromosome
• Accession: NZ_CP070048
• Organism: Escherichia coli strain FDAARGOS_1290

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: I6K20_RS05215
• Protein ID: WP_000813797.1
• Coordinates: 1022593..1022769 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K20_RS05220
• Protein ID: WP_076611057.1
• Coordinates: 1022815..1023231 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K20_RS05195 (1018215)	1018215..1019387	-	1173	WP_001236223.1	BenE family transporter YdcO	-
I6K20_RS05200 (1019479)	1019479..1020015	+	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
I6K20_RS05205 (1020088)	1020088..1022049	+	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K20_RS05210 (1022141)	1022141..1022371	-	231	WP_000494244.1	YncJ family protein	-
I6K20_RS05215 (1022593)	1022593..1022769	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K20_RS05220 (1022815)	1022815..1023231	+	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K20_RS05225 (1023310)	1023310..1024716	+	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
I6K20_RS05230 (1024961)	1024961..1026106	+	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
I6K20_RS05235 (1026124)	1026124..1027137	+	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
I6K20_RS05240 (1027138)	1027138..1028079	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T192829 WP_000813797.1 NZ_CP070048:1022593-1022769 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T192829 NZ_CP092556:c2103699-2103337 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT192829 WP_076611057.1 NZ_CP070048:1022815-1023231 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT192829 NZ_CP092556:c2103866-2103696 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A829J715