Detailed information of TA system TA192824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 626382..627166
• Replicon: chromosome
• Accession: NZ_CP070048
• Organism: Escherichia coli strain FDAARGOS_1290

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: I6K20_RS03105
• Protein ID: WP_000613626.1
• Coordinates: 626382..626876 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: I6K20_RS03110
• Protein ID: WP_001110447.1
• Coordinates: 626873..627166 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K20_RS03085 (621575)	621575..622672	+	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-
I6K20_RS03090 (622672)	622672..623613	+	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
I6K20_RS03095 (623679)	623679..625322	+	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
I6K20_RS03100 (625334)	625334..626287	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
I6K20_RS03105 (626382)	626382..626876	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
I6K20_RS03110 (626873)	626873..627166	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
I6K20_RS03115 (627299)	627299..630484	-	3186	WP_000827427.1	ribonuclease E	-
I6K20_RS03120 (631057)	631057..632016	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T192824 WP_000613626.1 NZ_CP070048:c626876-626382 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T192824 NZ_CP092554:c2499295-2499029 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT192824 WP_001110447.1 NZ_CP070048:c627166-626873 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT192824 NZ_CP092554:c2499552-2499295 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6