Detailed information of TA system TA192823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 379135..379840
• Replicon: chromosome
• Accession: NZ_CP070048
• Organism: Escherichia coli strain FDAARGOS_1290

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K20_RS01940
• Protein ID: WP_000539521.1
• Coordinates: 379454..379840 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K20_RS01935
• Protein ID: WP_001280945.1
• Coordinates: 379135..379464 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K20_RS01920 (374292)	374292..375203	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
I6K20_RS01925 (375381)	375381..377729	+	2349	WP_001322378.1	EAL domain-containing protein	-
I6K20_RS01930 (377737)	377737..379065	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
I6K20_RS01935 (379135)	379135..379464	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K20_RS01940 (379454)	379454..379840	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K20_RS01945 (380066)	380066..381391	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K20_RS01950 (381604)	381604..381987	+	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K20_RS01955 (382098)	382098..383213	+	1116	WP_109536249.1	aldose sugar dehydrogenase YliI	-
I6K20_RS01960 (383210)	383210..383836	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T192823 WP_000539521.1 NZ_CP070048:c379840-379454 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T192823 NZ_CP092554:c2442598-2442332 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT192823 WP_001280945.1 NZ_CP070048:c379464-379135 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT192823 NZ_CP092554:c2442849-2442598 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6