Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2347825..2348450 Replicon chromosome
Accession NZ_CP070046
Organism Klebsiella pneumoniae strain FDAARGOS_1319

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag I6K49_RS11395 Protein ID WP_004187928.1
Coordinates 2348067..2348450 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K49_RS11390 Protein ID WP_004150355.1
Coordinates 2347825..2348067 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K49_RS11365 2343827..2344426 + 600 WP_004151865.1 glucose-1-phosphatase -
I6K49_RS11370 2344420..2345280 + 861 WP_004146232.1 virulence factor BrkB family protein -
I6K49_RS11375 2345277..2345714 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K49_RS11380 2345759..2346700 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K49_RS11385 2346714..2347631 - 918 WP_004187929.1 alpha/beta hydrolase -
I6K49_RS11390 2347825..2348067 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K49_RS11395 2348067..2348450 + 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K49_RS11400 2348624..2349553 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6K49_RS11405 2349550..2350185 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K49_RS11410 2350182..2351084 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T192814 WP_004187928.1 NZ_CP070046:2348067-2348450 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T192814 NZ_CP092552:c2120908-2120699 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT192814 WP_004150355.1 NZ_CP070046:2347825-2348067 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT192814 NZ_CP092552:c2121213-2120920 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References