Detailed information of TA system TA192799

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5000467..5001302
• Replicon: chromosome
• Accession: NZ_CP070045
• Organism: Escherichia coli strain FDAARGOS_1303

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: I6K33_RS24820
• Protein ID: WP_001094426.1
• Coordinates: 5000925..5001302 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: I6K33_RS24815
• Protein ID: WP_001285575.1
• Coordinates: 5000467..5000835 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K33_RS24780 (4996360)	4996360..4997040	+	681	WP_001282919.1	WYL domain-containing protein	-
I6K33_RS24785 (4997188)	4997188..4997865	+	678	WP_001097301.1	hypothetical protein	-
I6K33_RS24790 (4997871)	4997871..4998104	+	234	WP_001278290.1	DUF905 family protein	-
I6K33_RS24795 (4998194)	4998194..4999012	+	819	WP_001175160.1	DUF932 domain-containing protein	-
I6K33_RS24800 (4999103)	4999103..4999588	+	486	WP_000213697.1	antirestriction protein	-
I6K33_RS24805 (4999603)	4999603..5000079	+	477	WP_001298074.1	RadC family protein	-
I6K33_RS24810 (5000166)	5000166..5000387	+	222	WP_000692350.1	DUF987 domain-containing protein	-
I6K33_RS24815 (5000467)	5000467..5000835	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K33_RS24820 (5000925)	5000925..5001302	+	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
I6K33_RS24825 (5001299)	5001299..5001787	+	489	WP_000761683.1	DUF5983 family protein	-
I6K33_RS24830 (5001799)	5001799..5001996	+	198	WP_000839272.1	DUF957 domain-containing protein	-
I6K33_RS24835 (5002081)	5002081..5002230	+	150	Protein_4798	hypothetical protein	-
I6K33_RS24840 (5003458)	5003458..5005221	+	1764	WP_001555776.1	hypothetical protein	-
I6K33_RS24845 (5005620)	5005620..5005799	-	180	Protein_4800	peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4987764..5015358	27594

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T192799 WP_001094426.1 NZ_CP070045:5000925-5001302 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T192799 NZ_CP092547:c2442809-2442543 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT192799 WP_001285575.1 NZ_CP070045:5000467-5000835 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT192799 NZ_CP092547:c2443060-2442809 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5