Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2240089..2240920 Replicon chromosome
Accession NZ_CP070045
Organism Escherichia coli strain FDAARGOS_1303

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K33_RS11605 Protein ID WP_000854814.1
Coordinates 2240546..2240920 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag I6K33_RS11600 Protein ID WP_001285586.1
Coordinates 2240089..2240457 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K33_RS11565 (2235147) 2235147..2236217 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
I6K33_RS11570 (2236353) 2236353..2237030 + 678 WP_001362823.1 hypothetical protein -
I6K33_RS11575 (2237046) 2237046..2237456 + 411 WP_000846704.1 hypothetical protein -
I6K33_RS11580 (2237677) 2237677..2238498 + 822 WP_001234570.1 DUF932 domain-containing protein -
I6K33_RS11585 (2238761) 2238761..2239234 + 474 WP_000855071.1 antirestriction protein -
I6K33_RS11590 (2239249) 2239249..2239725 + 477 WP_001298074.1 RadC family protein -
I6K33_RS11595 (2239794) 2239794..2240015 + 222 WP_000692323.1 DUF987 domain-containing protein -
I6K33_RS11600 (2240089) 2240089..2240457 + 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K33_RS11605 (2240546) 2240546..2240920 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K33_RS11610 (2240917) 2240917..2241111 + 195 WP_000988601.1 DUF5983 family protein -
I6K33_RS11615 (2241157) 2241157..2241237 + 81 Protein_2196 hypothetical protein -
I6K33_RS26140 (2241526) 2241526..2241606 - 81 WP_023441679.1 hypothetical protein -
I6K33_RS26145 (2241585) 2241585..2241908 + 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
I6K33_RS11630 (2242009) 2242009..2242338 - 330 WP_000450409.1 DUF496 family protein -
I6K33_RS11635 (2242510) 2242510..2243568 - 1059 WP_001200890.1 FUSC family protein -
I6K33_RS11640 (2243766) 2243766..2244239 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
I6K33_RS11645 (2244358) 2244358..2245523 - 1166 Protein_2202 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T192793 WP_000854814.1 NZ_CP070045:2240546-2240920 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T192793 NZ_CP092545:71533-71790 [Staphylococcus aureus]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT192793 WP_001285586.1 NZ_CP070045:2240089-2240457 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT192793 NZ_CP092545:71271-71540 [Staphylococcus aureus]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References