Detailed information of TA system TA192784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1685074..1686662
• Replicon: chromosome
• Accession: NZ_CP070045
• Organism: Escherichia coli strain FDAARGOS_1303

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6K33_RS08985
• Protein ID: WP_001125475.1
• Coordinates: 1685074..1686396 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K33_RS08990
• Protein ID: WP_001332145.1
• Coordinates: 1686396..1686662 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K33_RS08945 (1681072)	1681072..1681305	-	234	WP_000473544.1	hypothetical protein	-
I6K33_RS08950 (1681340)	1681340..1681591	-	252	WP_021579044.1	hypothetical protein	-
I6K33_RS08955 (1681594)	1681594..1681911	-	318	WP_000073835.1	DNA-binding protein	-
I6K33_RS08960 (1681923)	1681923..1682975	-	1053	WP_000568107.1	phage/plasmid replication protein, II/X family	-
I6K33_RS08965 (1683147)	1683147..1683593	+	447	WP_000527682.1	helix-turn-helix transcriptional regulator	-
I6K33_RS08970 (1683693)	1683693..1684115	+	423	WP_021579045.1	hypothetical protein	-
I6K33_RS08975 (1684144)	1684144..1684419	+	276	WP_000773941.1	hypothetical protein	-
I6K33_RS08980 (1684419)	1684419..1684898	+	480	WP_021579046.1	hypothetical protein	-
I6K33_RS08985 (1685074)	1685074..1686396	-	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K33_RS08990 (1686396)	1686396..1686662	-	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K33_RS08995 (1686970)	1686970..1687728	+	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6K33_RS09000 (1687732)	1687732..1688646	-	915	WP_001306069.1	bestrophin family protein	-
I6K33_RS09005 (1688843)	1688843..1690294	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T192784 WP_001125475.1 NZ_CP070045:c1686396-1685074 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192784 NZ_CP092542:c2472884-2472618 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT192784 WP_001332145.1 NZ_CP070045:c1686662-1686396 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT192784 NZ_CP092542:c2473141-2472884 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8