Detailed information of TA system TA192778

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1112530..1113328
• Replicon: chromosome
• Accession: NZ_CP070045
• Organism: Escherichia coli strain FDAARGOS_1303

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1RDD2
• Locus tag: I6K33_RS05850
• Protein ID: WP_001094434.1
• Coordinates: 1112954..1113328 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K33_RS05845
• Protein ID: WP_001552747.1
• Coordinates: 1112530..1112907 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K33_RS05815 (1109408)	1109408..1110229	+	822	WP_001234585.1	DUF932 domain-containing protein	-
I6K33_RS05820 (1110229)	1110229..1110474	+	246	WP_001164966.1	hypothetical protein	-
I6K33_RS05825 (1110568)	1110568..1111041	+	474	WP_001542276.1	antirestriction protein	-
I6K33_RS05830 (1111057)	1111057..1111533	+	477	WP_001186727.1	RadC family protein	-
I6K33_RS05835 (1111596)	1111596..1111817	+	222	WP_000692315.1	DUF987 domain-containing protein	-
I6K33_RS05840 (1111836)	1111836..1112480	+	645	WP_000086759.1	hypothetical protein	-
I6K33_RS05845 (1112530)	1112530..1112907	+	378	WP_001552747.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
I6K33_RS05850 (1112954)	1112954..1113328	+	375	WP_001094434.1	TA system toxin CbtA family protein	Toxin
I6K33_RS05855 (1113325)	1113325..1113816	+	492	WP_000976859.1	DUF5983 family protein	-
I6K33_RS05860 (1113828)	1113828..1114025	+	198	WP_000839257.1	DUF957 domain-containing protein	-
I6K33_RS05865 (1114118)	1114118..1114960	+	843	WP_001280460.1	DUF4942 domain-containing protein	-
I6K33_RS05875 (1115431)	1115431..1116369	+	939	WP_000351298.1	glyoxylate/hydroxypyruvate reductase GhrA	-
I6K33_RS05880 (1116424)	1116424..1117161	+	738	WP_000283664.1	zinc-binding phosphatase	-
I6K33_RS05885 (1117185)	1117185..1117739	+	555	WP_001001909.1	molecular chaperone YcdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13921.82 Da
• Isoelectric Point: 6.8517

>T192778 WP_001094434.1 NZ_CP070045:1112954-1113328 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T192778 NZ_CP092542:c825758-825570 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13757.63 Da
• Isoelectric Point: 6.3127

>AT192778 WP_001552747.1 NZ_CP070045:1112530-1112907 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRTGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT192778 NZ_CP092542:c826047-825787 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: Q1RDD2