Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 451788..452406 Replicon chromosome
Accession NZ_CP070045
Organism Escherichia coli strain FDAARGOS_1303

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K33_RS02645 Protein ID WP_001291435.1
Coordinates 451788..452006 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K33_RS02650 Protein ID WP_000344800.1
Coordinates 452032..452406 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K33_RS02610 (447075) 447075..447647 + 573 WP_000779831.1 YbaY family lipoprotein -
I6K33_RS02615 (447678) 447678..447989 - 312 WP_000409908.1 MGMT family protein -
I6K33_RS02625 (448368) 448368..448721 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K33_RS02630 (448763) 448763..450313 - 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K33_RS02635 (450477) 450477..450947 - 471 WP_000136192.1 YlaC family protein -
I6K33_RS02640 (451063) 451063..451614 - 552 WP_000102543.1 maltose O-acetyltransferase -
I6K33_RS02645 (451788) 451788..452006 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K33_RS02650 (452032) 452032..452406 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K33_RS02655 (452951) 452951..456100 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K33_RS02660 (456123) 456123..457316 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192776 WP_001291435.1 NZ_CP070045:c452006-451788 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192776 NZ_CP092540:c2499569-2499303 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192776 WP_000344800.1 NZ_CP070045:c452406-452032 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192776 NZ_CP092540:c2499826-2499569 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References