Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 70247..70772 Replicon plasmid unnamed1
Accession NZ_CP070042
Organism Escherichia coli strain FDAARGOS_1293

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag I6K23_RS24325 Protein ID WP_001159871.1
Coordinates 70467..70772 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6K23_RS24320 Protein ID WP_000813634.1
Coordinates 70247..70465 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K23_RS24315 (67284) 67284..67925 - 642 WP_000990667.1 hypothetical protein -
I6K23_RS24320 (70247) 70247..70465 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6K23_RS24325 (70467) 70467..70772 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
I6K23_RS24330 (70773) 70773..71582 + 810 WP_000016979.1 site-specific integrase -
I6K23_RS24335 (71720) 71720..71995 - 276 WP_000239529.1 hypothetical protein -
I6K23_RS24340 (71989) 71989..72633 - 645 WP_000633911.1 AAA family ATPase -
I6K23_RS24345 (72862) 72862..73833 + 972 WP_001103694.1 plasmid segregation protein ParM -
I6K23_RS24350 (73838) 73838..74230 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
I6K23_RS24355 (74235) 74235..75505 - 1271 Protein_77 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114229 114229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T192767 WP_001159871.1 NZ_CP070042:70467-70772 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T192767 NZ_CP092538:c2442451-2442185 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT192767 WP_000813634.1 NZ_CP070042:70247-70465 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT192767 NZ_CP092538:c2442702-2442451 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References