Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4184827..4185481 Replicon chromosome
Accession NZ_CP070041
Organism Escherichia coli strain FDAARGOS_1293

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K23_RS20190 Protein ID WP_000244781.1
Coordinates 4185074..4185481 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K23_RS20185 Protein ID WP_000354046.1
Coordinates 4184827..4185093 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K23_RS20165 (4180915) 4180915..4182348 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
I6K23_RS20170 (4182393) 4182393..4182704 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6K23_RS20175 (4182868) 4182868..4183527 + 660 WP_000250274.1 hemolysin III family protein -
I6K23_RS20180 (4183604) 4183604..4184584 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
I6K23_RS20185 (4184827) 4184827..4185093 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K23_RS20190 (4185074) 4185074..4185481 + 408 WP_000244781.1 protein YgfX Toxin
I6K23_RS20195 (4185521) 4185521..4186042 - 522 WP_001055874.1 flavodoxin FldB -
I6K23_RS20200 (4186154) 4186154..4187050 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6K23_RS20205 (4187075) 4187075..4187785 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K23_RS20210 (4187791) 4187791..4189524 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T192765 WP_000244781.1 NZ_CP070041:4185074-4185481 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192765 NZ_CP092538:c2102681-2102319 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192765 WP_000354046.1 NZ_CP070041:4184827-4185093 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192765 NZ_CP092538:c2102848-2102678 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References