Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2288263..2288813 Replicon chromosome
Accession NZ_CP070041
Organism Escherichia coli strain FDAARGOS_1293

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6K23_RS11110 Protein ID WP_001160966.1
Coordinates 2288263..2288577 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6K23_RS11115 Protein ID WP_000125566.1
Coordinates 2288580..2288813 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K23_RS11085 (2283938) 2283938..2285224 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
I6K23_RS11090 (2285224) 2285224..2286213 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6K23_RS11095 (2286210) 2286210..2287031 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6K23_RS11100 (2287034) 2287034..2287411 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6K23_RS11105 (2287418) 2287418..2288266 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6K23_RS11110 (2288263) 2288263..2288577 - 315 WP_001160966.1 CcdB family protein Toxin
I6K23_RS11115 (2288580) 2288580..2288813 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6K23_RS11120 (2288899) 2288899..2289378 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6K23_RS11125 (2289570) 2289570..2291432 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
I6K23_RS11130 (2291425) 2291425..2291955 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6K23_RS11135 (2292063) 2292063..2293394 - 1332 WP_001183207.1 MFS transporter -
I6K23_RS11140 (2293453) 2293453..2293740 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T192756 WP_001160966.1 NZ_CP070041:c2288577-2288263 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T192756 NZ_CP092536:c4706280-4705747 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT192756 WP_000125566.1 NZ_CP070041:c2288813-2288580 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT192756 NZ_CP092536:c4706557-4706291 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAGTGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References