Detailed information of TA system TA192755

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2087888..2088686
• Replicon: chromosome
• Accession: NZ_CP070041
• Organism: Escherichia coli strain FDAARGOS_1293

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VRA6
• Locus tag: I6K23_RS10170
• Protein ID: WP_000854730.1
• Coordinates: 2088309..2088686 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P0SQV8
• Locus tag: I6K23_RS10165
• Protein ID: WP_001285481.1
• Coordinates: 2087888..2088262 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K23_RS10125 (2083863)	2083863..2084315	+	453	WP_000682723.1	hypothetical protein	-
I6K23_RS10130 (2084433)	2084433..2084666	+	234	WP_001213776.1	DUF905 family protein	-
I6K23_RS10135 (2084766)	2084766..2085587	+	822	WP_001234359.1	DUF932 domain-containing protein	-
I6K23_RS10140 (2085587)	2085587..2085832	+	246	WP_001164966.1	hypothetical protein	-
I6K23_RS10145 (2085926)	2085926..2086399	+	474	WP_001313575.1	antirestriction protein	-
I6K23_RS10150 (2086415)	2086415..2086891	+	477	WP_001313574.1	RadC family protein	-
I6K23_RS10155 (2086954)	2086954..2087175	+	222	WP_000692315.1	DUF987 domain-containing protein	-
I6K23_RS10160 (2087194)	2087194..2087838	+	645	WP_000086761.1	hypothetical protein	-
I6K23_RS10165 (2087888)	2087888..2088262	+	375	WP_001285481.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K23_RS10170 (2088309)	2088309..2088686	+	378	WP_000854730.1	TA system toxin CbtA family protein	Toxin
I6K23_RS10175 (2088683)	2088683..2089175	+	493	Protein_2014	DUF5983 family protein	-
I6K23_RS10180 (2089254)	2089254..2090242	-	989	Protein_2015	IS630 family transposase	-
I6K23_RS10185 (2090390)	2090390..2090578	-	189	Protein_2016	IS66 family transposase	-
I6K23_RS10190 (2090639)	2090639..2091772	+	1134	WP_000555401.1	IS110-like element ISEc45 family transposase	-
I6K23_RS10195 (2092026)	2092026..2093430	-	1405	Protein_2018	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14212.21 Da
• Isoelectric Point: 7.2923

>T192755 WP_000854730.1 NZ_CP070041:2088309-2088686 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T192755 NZ_CP092536:4101990-4102208 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.53 Da
• Isoelectric Point: 4.7511

>AT192755 WP_001285481.1 NZ_CP070041:2087888-2088262 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT192755 NZ_CP092536:4101589-4101963 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0H2V671

Antitoxin

• AlphaFold DB: A0A0P0SQV8