Detailed information of TA system TA192751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 716342..717930
• Replicon: chromosome
• Accession: NZ_CP070041
• Organism: Escherichia coli strain FDAARGOS_1293

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1L1KDK2
• Locus tag: I6K23_RS03455
• Protein ID: WP_001125431.1
• Coordinates: 716608..717930 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: I6K23_RS03450
• Protein ID: WP_001304368.1
• Coordinates: 716342..716608 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K23_RS03435 (712710)	712710..714161	+	1452	WP_000854660.1	tagaturonate reductase	-
I6K23_RS03440 (714358)	714358..715272	+	915	WP_001443133.1	bestrophin family protein	-
I6K23_RS03445 (715276)	715276..716034	-	759	WP_001286540.1	trans-aconitate 2-methyltransferase	-
I6K23_RS03450 (716342)	716342..716608	+	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K23_RS03455 (716608)	716608..717930	+	1323	WP_001125431.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K23_RS03460 (718240)	718240..718647	-	408	Protein_685	acetylxylan esterase	-
I6K23_RS03465 (718650)	718650..719231	-	582	Protein_686	LysR family transcriptional regulator	-
I6K23_RS03470 (719418)	719418..719849	+	432	Protein_687	fimbria/pilus periplasmic chaperone	-
I6K23_RS03475 (719891)	719891..722542	+	2652	WP_000123643.1	fimbria/pilus outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49310.02 Da
• Isoelectric Point: 8.6972

>T192751 WP_001125431.1 NZ_CP070041:716608-717930 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192751 NZ_CP092536:359629-359976 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT192751 WP_001304368.1 NZ_CP070041:716342-716608 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT192751 NZ_CP092536:359976-360275 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A1L1KDK2

Antitoxin

• AlphaFold DB: V0VAH2