Detailed information of TA system TA192740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 90459..91102
• Replicon: plasmid unnamed2
• Accession: NZ_CP070039
• Organism: Klebsiella pneumoniae strain FDAARGOS_1322

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: I6K52_RS26515
• Protein ID: WP_001044770.1
• Coordinates: 90459..90875 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: I6K52_RS26520
• Protein ID: WP_001261282.1
• Coordinates: 90872..91102 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K52_RS26480	85829..86164	-	336	WP_000046891.1	thermonuclease family protein	-
I6K52_RS26485	86407..86970	+	564	WP_001515734.1	transposase	-
I6K52_RS26490	87035..87409	+	375	WP_001348195.1	cupin domain-containing protein	-
I6K52_RS26495	87433..87996	+	564	WP_001097412.1	chlorite dismutase family protein	-
I6K52_RS26500	88247..89344	-	1098	Protein_91	IS66 family transposase	-
I6K52_RS26505	89398..90102	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I6K52_RS26510	90149..90385	-	237	WP_001348075.1	AAA family ATPase	-
I6K52_RS26515	90459..90875	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
I6K52_RS26520	90872..91102	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6K52_RS26525	91059..91520	+	462	WP_167876147.1	hypothetical protein	-
I6K52_RS26530	91664..92014	+	351	WP_040119804.1	hypothetical protein	-
I6K52_RS26535	92065..92808	+	744	WP_000129823.1	hypothetical protein	-
I6K52_RS26540	92805..93581	+	777	WP_000015958.1	site-specific integrase	-
I6K52_RS26545	93639..93896	-	258	WP_000764642.1	hypothetical protein	-
I6K52_RS26550	94025..94129	-	105	WP_032409716.1	hypothetical protein	-
I6K52_RS26555	94664..95530	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	formA	-	1..107040	107040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T192740 WP_001044770.1 NZ_CP070039:c90875-90459 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T192740 NZ_CP092534:c3492528-3492226 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT192740 WP_001261282.1 NZ_CP070039:c91102-90872 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT192740 NZ_CP092534:c3492190-3491849 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3