Detailed information of TA system TA192728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3354084..3354670
• Replicon: chromosome
• Accession: NZ_CP070037
• Organism: Klebsiella pneumoniae strain FDAARGOS_1322

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A486RB10
• Locus tag: I6K52_RS16170
• Protein ID: WP_004174007.1
• Coordinates: 3354302..3354670 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: I6K52_RS16165
• Protein ID: WP_004174006.1
• Coordinates: 3354084..3354305 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K52_RS16145	3350241..3351167	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
I6K52_RS16150	3351164..3352441	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
I6K52_RS16155	3352438..3353205	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
I6K52_RS16160	3353207..3353920	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
I6K52_RS16165	3354084..3354305	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6K52_RS16170	3354302..3354670	+	369	WP_004174007.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
I6K52_RS16175	3354943..3356259	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
I6K52_RS16180	3356366..3357253	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
I6K52_RS16185	3357250..3358095	+	846	WP_004174009.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
I6K52_RS16190	3358097..3359167	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3349070..3359904	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-116)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13568.97 Da
• Isoelectric Point: 8.6410

>T192728 WP_004174007.1 NZ_CP070037:3354302-3354670 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDLGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T192728 NZ_CP092533:4406476-4406850 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT192728 WP_004174006.1 NZ_CP070037:3354084-3354305 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT192728 NZ_CP092533:4406018-4406386 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A486RB10

Antitoxin

• AlphaFold DB: A0A5E5YJY7