Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2950294..2950919 Replicon chromosome
Accession NZ_CP070037
Organism Klebsiella pneumoniae strain FDAARGOS_1322

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I6K52_RS14290 Protein ID WP_002882817.1
Coordinates 2950294..2950677 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I6K52_RS14295 Protein ID WP_004150355.1
Coordinates 2950677..2950919 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K52_RS14275 2947660..2948562 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I6K52_RS14280 2948559..2949194 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I6K52_RS14285 2949191..2950120 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I6K52_RS14290 2950294..2950677 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I6K52_RS14295 2950677..2950919 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I6K52_RS14300 2951124..2952041 + 918 WP_135726069.1 alpha/beta hydrolase -
I6K52_RS14305 2952055..2952996 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I6K52_RS14310 2953041..2953478 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I6K52_RS14315 2953475..2954335 - 861 WP_004146232.1 virulence factor BrkB family protein -
I6K52_RS14320 2954329..2954928 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T192727 WP_002882817.1 NZ_CP070037:c2950677-2950294 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T192727 NZ_CP092533:4147785-4148042 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT192727 WP_004150355.1 NZ_CP070037:c2950919-2950677 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT192727 NZ_CP092533:4148054-4148599 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCGGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References