Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 2395019..2395710 Replicon chromosome
Accession NZ_CP070037
Organism Klebsiella pneumoniae strain FDAARGOS_1322

Toxin (Protein)


Gene name ykfI Uniprot ID A0A483YFJ1
Locus tag I6K52_RS11680 Protein ID WP_025987721.1
Coordinates 2395019..2395360 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2A5MHA6
Locus tag I6K52_RS11685 Protein ID WP_019725272.1
Coordinates 2395384..2395710 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K52_RS11645 2390242..2390472 - 231 WP_077256410.1 hypothetical protein -
I6K52_RS11650 2390541..2390729 - 189 WP_077256411.1 hypothetical protein -
I6K52_RS11655 2391182..2391874 - 693 WP_040173594.1 HNH endonuclease -
I6K52_RS11660 2392071..2392653 + 583 Protein_2289 tyrosine-type recombinase/integrase -
I6K52_RS11665 2392794..2393702 + 909 WP_077256408.1 hypothetical protein -
I6K52_RS11670 2393781..2394065 + 285 Protein_2291 helix-turn-helix domain-containing protein -
I6K52_RS11675 2394071..2394904 - 834 WP_040173641.1 DUF4942 domain-containing protein -
I6K52_RS11680 2395019..2395360 - 342 WP_025987721.1 TA system toxin CbtA family protein Toxin
I6K52_RS11685 2395384..2395710 - 327 WP_019725272.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
I6K52_RS11690 2395724..2396200 - 477 WP_019725273.1 DNA repair protein RadC -
I6K52_RS11695 2396210..2396656 - 447 WP_040173599.1 antirestriction protein -
I6K52_RS11700 2396867..2397556 - 690 WP_040173600.1 hypothetical protein -
I6K52_RS11705 2398121..2399011 - 891 WP_040173607.1 50S ribosome-binding GTPase -
I6K52_RS11710 2399094..2400182 - 1089 WP_085163515.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2385241..2416874 31633


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(8-107)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12867.90 Da        Isoelectric Point: 8.5195

>T192724 WP_025987721.1 NZ_CP070037:c2395360-2395019 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Download         Length: 342 bp

>T192724 NZ_CP092533:c3994390-3994076 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12276.01 Da        Isoelectric Point: 6.9560

>AT192724 WP_019725272.1 NZ_CP070037:c2395710-2395384 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Download         Length: 327 bp

>AT192724 NZ_CP092533:c3994626-3994393 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YFJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MHA6

References