Detailed information of TA system TA192721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 419730..421318
• Replicon: chromosome
• Accession: NZ_CP070037
• Organism: Klebsiella pneumoniae strain FDAARGOS_1322

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I6K52_RS02130
• Protein ID: WP_135725863.1
• Coordinates: 419730..421052 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A483IVM3
• Locus tag: I6K52_RS02135
• Protein ID: WP_029496892.1
• Coordinates: 421052..421318 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K52_RS02115	414786..417287	+	2502	WP_032410312.1	fimbrial biogenesis outer membrane usher protein	-
I6K52_RS02120	417275..418279	+	1005	WP_009485243.1	type 1 fimbrial protein	-
I6K52_RS02125	418421..419713	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
I6K52_RS02130	419730..421052	-	1323	WP_135725863.1	type II toxin-antitoxin system HipA family toxin	Toxin
I6K52_RS02135	421052..421318	-	267	WP_029496892.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K52_RS02140	421705..422031	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
I6K52_RS02145	422028..422528	-	501	WP_020804988.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I6K52_RS02150	422506..423885	-	1380	WP_020804975.1	purine permease	-
I6K52_RS02155	424232..425386	+	1155	WP_020804972.1	FAD-dependent urate hydroxylase HpxO	-
I6K52_RS02160	425367..426290	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-64)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49110.47 Da
• Isoelectric Point: 7.5366

>T192721 WP_135725863.1 NZ_CP070037:c421052-419730 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPLVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T192721 NZ_CP092533:c3492503-3492201 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9938.33 Da
• Isoelectric Point: 8.5912

>AT192721 WP_029496892.1 NZ_CP070037:c421318-421052 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGMVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT192721 NZ_CP092533:c3492165-3491824 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A483IVM3