Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 47433..48034 Replicon plasmid unnamed1
Accession NZ_CP070027
Organism Escherichia coli strain FDAARGOS_1337

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag I6K67_RS23255 Protein ID WP_001216045.1
Coordinates 47433..47813 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag I6K67_RS23260 Protein ID WP_001190712.1
Coordinates 47813..48034 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K67_RS23220 (I6K67_23220) 42562..43413 + 852 WP_000611664.1 phage repressor protein C1 -
I6K67_RS24360 43446..43778 - 333 WP_224205061.1 phage antirepressor KilAC domain-containing protein -
I6K67_RS24365 43899..44249 - 351 Protein_44 ORF6N domain-containing protein -
I6K67_RS23230 (I6K67_23230) 44558..44779 - 222 WP_001361625.1 host cell division inhibitor Icd-like protein -
I6K67_RS23235 (I6K67_23235) 44798..44917 - 120 WP_071527722.1 ash family protein -
I6K67_RS23240 (I6K67_23240) 45086..46054 + 969 WP_000654811.1 IS5 family transposase -
I6K67_RS23245 (I6K67_23245) 46178..47221 - 1044 WP_072647226.1 DUF968 domain-containing protein -
I6K67_RS23250 (I6K67_23250) 47249..47428 - 180 WP_000113018.1 hypothetical protein -
I6K67_RS23255 (I6K67_23255) 47433..47813 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I6K67_RS23260 (I6K67_23260) 47813..48034 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6K67_RS23265 (I6K67_23265) 48217..49773 + 1557 WP_072647227.1 type I restriction-modification system subunit M -
I6K67_RS23270 (I6K67_23270) 49770..51038 + 1269 WP_072647224.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..110044 110044
- flank IS/Tn - - 45086..46054 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T192719 WP_001216045.1 NZ_CP070027:c47813-47433 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T192719 NZ_CP092533:2450582-2450860 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT192719 WP_001190712.1 NZ_CP070027:c48034-47813 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT192719 NZ_CP092533:2450860-2451144 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References