Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2316417..2317035 Replicon chromosome
Accession NZ_CP070026
Organism Escherichia coli strain FDAARGOS_1337

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K67_RS11345 Protein ID WP_001291435.1
Coordinates 2316417..2316635 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K67_RS11350 Protein ID WP_000344800.1
Coordinates 2316661..2317035 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K67_RS11310 (2311706) 2311706..2312278 + 573 WP_000779831.1 YbaY family lipoprotein -
I6K67_RS11315 (2312309) 2312309..2312620 - 312 WP_000409911.1 MGMT family protein -
I6K67_RS11325 (2312999) 2312999..2313352 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K67_RS11330 (2313394) 2313394..2314944 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K67_RS11335 (2315108) 2315108..2315578 - 471 WP_000136192.1 YlaC family protein -
I6K67_RS11340 (2315694) 2315694..2316245 - 552 WP_000102564.1 maltose O-acetyltransferase -
I6K67_RS11345 (2316417) 2316417..2316635 - 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K67_RS11350 (2316661) 2316661..2317035 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K67_RS11355 (2317581) 2317581..2320730 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K67_RS11360 (2320753) 2320753..2321946 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192701 WP_001291435.1 NZ_CP070026:c2316635-2316417 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192701 NZ_CP092532:2737057-2737164 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192701 WP_000344800.1 NZ_CP070026:c2317035-2316661 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192701 NZ_CP092532:c2737004-2736943 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References