Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 149110..149764 Replicon chromosome
Accession NZ_CP070026
Organism Escherichia coli strain FDAARGOS_1337

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag I6K67_RS01025 Protein ID WP_204691588.1
Coordinates 149110..149517 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K67_RS01030 Protein ID WP_000354046.1
Coordinates 149498..149764 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K67_RS01005 (145067) 145067..146800 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
I6K67_RS01010 (146806) 146806..147516 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K67_RS01015 (147541) 147541..148437 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6K67_RS01020 (148549) 148549..149070 + 522 WP_001055874.1 flavodoxin FldB -
I6K67_RS01025 (149110) 149110..149517 - 408 WP_204691588.1 protein YgfX Toxin
I6K67_RS01030 (149498) 149498..149764 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K67_RS01035 (150007) 150007..150987 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I6K67_RS01040 (151183) 151183..151842 - 660 WP_000250274.1 hemolysin III family protein -
I6K67_RS01045 (152006) 152006..152317 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
I6K67_RS01050 (152362) 152362..153795 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
I6K67_RS01055 (153852) 153852..154595 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T192694 WP_204691588.1 NZ_CP070026:c149517-149110 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSCMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192694 NZ_CP092532:726259-726555 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192694 WP_000354046.1 NZ_CP070026:c149764-149498 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192694 NZ_CP092532:726557-726952 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References