Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 37007..37743 Replicon plasmid unnamed1
Accession NZ_CP070021
Organism Klebsiella oxytoca strain FDAARGOS_1333

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag I6K63_RS27355 Protein ID WP_003026803.1
Coordinates 37007..37489 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I6K63_RS27360 Protein ID WP_003026799.1
Coordinates 37477..37743 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K63_RS27335 32557..33519 + 963 WP_004152113.1 M48 family metalloprotease -
I6K63_RS27340 33607..34861 + 1255 Protein_36 IS3 family transposase -
I6K63_RS27345 35009..35404 - 396 WP_004143398.1 helix-turn-helix domain-containing protein -
I6K63_RS27350 35453..36799 - 1347 WP_077253535.1 ISNCY family transposase -
I6K63_RS27355 37007..37489 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
I6K63_RS27360 37477..37743 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I6K63_RS27365 37919..38173 - 255 WP_004152108.1 hypothetical protein -
I6K63_RS27370 38249..38506 - 258 WP_004152107.1 hypothetical protein -
I6K63_RS27375 38555..38758 - 204 WP_004152106.1 hemolysin expression modulator Hha -
I6K63_RS27380 38792..39160 - 369 WP_004152105.1 hypothetical protein -
I6K63_RS27385 39204..39698 - 495 WP_004152104.1 DNA-binding protein -
I6K63_RS27390 39729..40304 - 576 WP_004152103.1 hypothetical protein -
I6K63_RS27395 40292..40561 - 270 WP_004152102.1 hypothetical protein -
I6K63_RS27400 40919..41269 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
I6K63_RS27405 41319..41681 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..176321 176321


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T192691 WP_003026803.1 NZ_CP070021:c37489-37007 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T192691 NZ_CP092524:5134-5406 [Cetobacterium somerae]
ATGATTAAATACAGAGTTGAATTTTCAGAAACATCTTTAGGACATTTAAAAAAATTAGATAAATCAGTTCAGACTATTAT
TTTAAAATGGATTAAAAAAAATCTTGTTGATTGTAATAATCCTAAAGCACATGGAAAAGCATTAGTAGGAAATAAAAAAG
GATTTTGGCGATACAGAGTAGGTAATTATAGATTACTTTGTAATATCCATGATGAGGTATTAACTATATTAGTTCTTGAA
GTAGGACATAGAAGTGAAATTTATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT192691 WP_003026799.1 NZ_CP070021:c37743-37477 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT192691 NZ_CP092524:4907-5137 [Cetobacterium somerae]
ATGTCAATTATTTCTGTTAGATTAAATACTGATGAAGAGCTATTATTAAAAAAAGTTGCTGATTTTGAAGGTGTTGGACT
TTCTAGCTATATAAAAAAAATTGTTTTTGAAAGATTGGAAGAAGAATATGATTTAAAAGTTGCTGAAAATGCTTATAAAA
CTCATGTAGAATCAAACCAAAAAACTTACTCTTTTGATAGTGTAATTGAGGAATTAGGAATTGATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References