Detailed information of TA system TA192681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1277956..1278575
• Replicon: chromosome
• Accession: NZ_CP070020
• Organism: Klebsiella oxytoca strain FDAARGOS_1333

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: I6K63_RS05885
• Protein ID: WP_004099646.1
• Coordinates: 1277956..1278174 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: I6K63_RS05890
• Protein ID: WP_004099648.1
• Coordinates: 1278201..1278575 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K63_RS05855	1273993..1274256	+	264	WP_123830207.1	type B 50S ribosomal protein L31	-
I6K63_RS05860	1274256..1274396	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
I6K63_RS05865	1274393..1275091	-	699	WP_004099639.1	GNAT family N-acetyltransferase	-
I6K63_RS05870	1275193..1276647	+	1455	WP_196557705.1	PLP-dependent aminotransferase family protein	-
I6K63_RS05875	1276622..1277092	-	471	WP_004111038.1	YlaC family protein	-
I6K63_RS05880	1277229..1277795	-	567	WP_004099644.1	maltose O-acetyltransferase	-
I6K63_RS05885	1277956..1278174	-	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
I6K63_RS05890	1278201..1278575	-	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
I6K63_RS05895	1279057..1282203	-	3147	WP_196557707.1	multidrug efflux RND transporter permease subunit AcrB	-
I6K63_RS05900	1282226..1283419	-	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T192681 WP_004099646.1 NZ_CP070020:c1278174-1277956 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T192681 NZ_CP092511:31322-31624 [Salmonella enterica subsp. enterica serovar Thompson]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT192681 WP_004099648.1 NZ_CP070020:c1278575-1278201 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT192681 NZ_CP092511:31695-32033 [Salmonella enterica subsp. enterica serovar Thompson]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0H3H713