Detailed information of TA system TA192680

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 1033008..1033725
• Replicon: chromosome
• Accession: NZ_CP070020
• Organism: Klebsiella oxytoca strain FDAARGOS_1333

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: I6K63_RS04725
• Protein ID: WP_071994601.1
• Coordinates: 1033008..1033340 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8B4G4X8
• Locus tag: I6K63_RS04730
• Protein ID: WP_032410127.1
• Coordinates: 1033375..1033725 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K63_RS04710	1028538..1028750	-	213	WP_196558232.1	KTSC domain-containing protein	-
I6K63_RS04715	1029528..1030310	-	783	Protein_906	IS4 family transposase	-
I6K63_RS04720	1030862..1032007	+	1146	WP_196558258.1	TIR domain-containing protein	-
I6K63_RS04725	1033008..1033340	-	333	WP_071994601.1	TA system toxin CbtA family protein	Toxin
I6K63_RS04730	1033375..1033725	-	351	WP_032410127.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K63_RS04735	1033746..1033967	-	222	WP_004129348.1	DUF987 domain-containing protein	-
I6K63_RS04740	1033983..1034456	-	474	WP_032410128.1	DNA repair protein RadC	-
I6K63_RS04745	1034527..1035348	-	822	WP_162897387.1	DUF945 domain-containing protein	-
I6K63_RS04750	1035444..1036121	-	678	WP_032410130.1	hypothetical protein	-
I6K63_RS04755	1036405..1037298	-	894	WP_032410131.1	50S ribosome-binding GTPase	-
I6K63_RS04760	1037396..1038547	+	1152	WP_196558252.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1028538..1058911	30373
	-	flank	IS/Tn	-	-	1029528..1030160	632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (15-115)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12513.46 Da
• Isoelectric Point: 7.2132

>T192680 WP_071994601.1 NZ_CP070020:c1033340-1033008 [Klebsiella oxytoca]
MNTLPAINQRAVQTCLSPVVVWQMLLARLLEQHYGLTLNDTPFSDEAVLKRHIEAGITLVDAVNFLVEKYELIRIDRRGF
SSQGQVPYLTVTDILHARRECGLTNSCPYR

Nucleotide

• Length: 333 bp

>T192680 NZ_CP070020:c1033340-1033008 [Klebsiella oxytoca]
ATGAATACTTTACCTGCAATAAACCAGCGGGCTGTTCAGACATGCTTATCACCCGTGGTTGTCTGGCAAATGCTGCTTGC
TCGACTACTTGAGCAGCACTATGGCCTGACCTTAAATGACACGCCGTTCAGCGATGAGGCTGTTCTTAAAAGACATATCG
AAGCTGGTATCACGCTTGTCGATGCCGTTAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGATCGCAGAGGATTT
AGCTCACAGGGACAAGTGCCATATTTGACCGTGACAGATATCCTTCATGCCAGGAGAGAATGTGGATTAACGAACAGTTG
TCCTTATCGGTAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13014.93 Da
• Isoelectric Point: 7.1865

>AT192680 WP_032410127.1 NZ_CP070020:c1033725-1033375 [Klebsiella oxytoca]
MSSKTLTVNDDTAEPWWGLNRNVIPCFGARLVQEGNRLYYLSDRASIIGLFNKADLLRLDQAFPVLLKQAELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSCGHVYISIYPTQR

Nucleotide

• Length: 351 bp

>AT192680 NZ_CP070020:c1033725-1033375 [Klebsiella oxytoca]
ATGTCGAGTAAGACACTCACAGTGAATGATGATACCGCTGAGCCCTGGTGGGGGCTGAATCGTAATGTCATTCCTTGTTT
TGGTGCGCGTCTGGTCCAGGAAGGTAACCGCCTATATTACCTGTCTGACCGCGCCAGCATAATAGGCCTATTCAATAAAG
CGGATTTGCTCCGTCTTGACCAGGCATTTCCTGTTCTGCTGAAGCAGGCGGAGCTCATGCTTACCAGCGGCGAACTGAAT
CCCCGACACCAGCATTGCGTCACCCTCTATGCAAAGGGGCTGACCTGCGAAGCTGATACACTGGGTTCCTGTGGTCACGT
CTATATCTCCATTTATCCTACTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B4G4X8