Detailed information of TA system TA192659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 5331..5796
• Replicon: plasmid unnamed5
• Accession: NZ_CP070012
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: I6K18_RS27865
• Protein ID: WP_001230707.1
• Coordinates: 5545..5796 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: I6K18_RS27860
• Protein ID: WP_001541977.1
• Coordinates: 5331..5558 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS27820 (I6K18_27820)	506..772	-	267	WP_000160396.1	hypothetical protein	-
I6K18_RS27825 (I6K18_27825)	849..2078	-	1230	WP_001694183.1	DNA relaxase/nickase TaxC	-
I6K18_RS27830 (I6K18_27830)	2090..2590	-	501	WP_001694182.1	DNA distortion polypeptide 1	-
I6K18_RS27835 (I6K18_27835)	2951..3298	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
I6K18_RS27840 (I6K18_27840)	3295..3543	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
I6K18_RS27845 (I6K18_27845)	3848..4141	-	294	WP_032233324.1	hypothetical protein	-
I6K18_RS27850 (I6K18_27850)	4156..4530	-	375	WP_001473357.1	hypothetical protein	-
I6K18_RS27855 (I6K18_27855)	4583..4882	-	300	WP_000650311.1	hypothetical protein	-
I6K18_RS27860 (I6K18_27860)	5331..5558	-	228	WP_001541977.1	hypothetical protein	Antitoxin
I6K18_RS27865 (I6K18_27865)	5545..5796	-	252	WP_001230707.1	hypothetical protein	Toxin
I6K18_RS27870 (I6K18_27870)	5793..6335	-	543	WP_001083906.1	hypothetical protein	-
I6K18_RS27875 (I6K18_27875)	6440..6955	-	516	WP_001025395.1	J domain-containing protein	-
I6K18_RS27880 (I6K18_27880)	8364..9230	+	867	WP_050557380.1	replication initiation protein	-
I6K18_RS27885 (I6K18_27885)	9243..9647	+	405	WP_170997721.1	hypothetical protein	-
I6K18_RS27890 (I6K18_27890)	9782..10309	+	528	WP_021534055.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32611	32611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T192659 WP_001230707.1 NZ_CP070012:c5796-5545 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T192659 NZ_CP092500:2181313-2182635 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAAAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGCTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGATAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAACAGCTCACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTAGGCATGATTAGACAAGAAAATGACTTTCGCATCTCGGTTGCTGGAGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATTACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGACCAGGCATCGCGCGGATCATGACCTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGCTGCACCGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.00 Da
• Isoelectric Point: 9.1450

>AT192659 WP_001541977.1 NZ_CP070012:c5558-5331 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRQSK

Nucleotide

• Length: 228 bp

>AT192659 NZ_CP092500:2181047-2181313 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: U9ZUY2