Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2951..3543 Replicon plasmid unnamed5
Accession NZ_CP070012
Organism Escherichia coli strain FDAARGOS_1288

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag I6K18_RS27840 Protein ID WP_000520549.1
Coordinates 3295..3543 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag I6K18_RS27835 Protein ID WP_000681613.1
Coordinates 2951..3298 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K18_RS27815 (I6K18_27815) 268..501 - 234 WP_000121162.1 EexN family lipoprotein -
I6K18_RS27820 (I6K18_27820) 506..772 - 267 WP_000160396.1 hypothetical protein -
I6K18_RS27825 (I6K18_27825) 849..2078 - 1230 WP_001694183.1 DNA relaxase/nickase TaxC -
I6K18_RS27830 (I6K18_27830) 2090..2590 - 501 WP_001694182.1 DNA distortion polypeptide 1 -
I6K18_RS27835 (I6K18_27835) 2951..3298 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I6K18_RS27840 (I6K18_27840) 3295..3543 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
I6K18_RS27845 (I6K18_27845) 3848..4141 - 294 WP_032233324.1 hypothetical protein -
I6K18_RS27850 (I6K18_27850) 4156..4530 - 375 WP_001473357.1 hypothetical protein -
I6K18_RS27855 (I6K18_27855) 4583..4882 - 300 WP_000650311.1 hypothetical protein -
I6K18_RS27860 (I6K18_27860) 5331..5558 - 228 WP_001541977.1 hypothetical protein -
I6K18_RS27865 (I6K18_27865) 5545..5796 - 252 WP_001230707.1 hypothetical protein -
I6K18_RS27870 (I6K18_27870) 5793..6335 - 543 WP_001083906.1 hypothetical protein -
I6K18_RS27875 (I6K18_27875) 6440..6955 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..32611 32611


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T192658 WP_000520549.1 NZ_CP070012:c3543-3295 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T192658 NZ_CP092500:1840590-1840693 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT192658 WP_000681613.1 NZ_CP070012:c3298-2951 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT192658 NZ_CP092500:c1840731-1840463 [Escherichia coli]
AAAGTCAGCGAAGGGAATGTTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References