Detailed information of TA system TA192653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 130484..131043
• Replicon: plasmid unnamed1
• Accession: NZ_CP070008
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: I6K18_RS26375
• Protein ID: WP_000421272.1
• Coordinates: 130484..130759 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: I6K18_RS26380
• Protein ID: WP_001387467.1
• Coordinates: 130759..131043 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS26345 (126521)	126521..127261	-	741	WP_001066953.1	tyrosine-type recombinase/integrase	-
I6K18_RS26350 (127382)	127382..127564	-	183	WP_042344623.1	hypothetical protein	-
I6K18_RS28450 (127631)	127631..127920	-	290	Protein_149	type II toxin-antitoxin system RelE/ParE family toxin	-
I6K18_RS26355 (127907)	127907..128175	-	269	Protein_150	type II toxin-antitoxin system ParD family antitoxin	-
I6K18_RS26360 (128707)	128707..129900	+	1194	WP_251301810.1	hypothetical protein	-
I6K18_RS26365 (129922)	129922..130050	+	129	Protein_152	IS481 family transposase	-
I6K18_RS26370 (130175)	130175..130378	-	204	Protein_153	hypothetical protein	-
I6K18_RS26375 (130484)	130484..130759	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K18_RS26380 (130759)	130759..131043	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6K18_RS26385 (131648)	131648..132400	+	753	WP_000562172.1	IclR family transcriptional regulator	-
I6K18_RS26390 (132446)	132446..133411	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
I6K18_RS26395 (133444)	133444..133824	-	381	WP_000710783.1	Rid family detoxifying hydrolase	-
I6K18_RS26400 (133849)	133849..134739	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
I6K18_RS26405 (135140)	135140..135580	+	441	Protein_160	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA14 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..198318	198318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T192653 WP_000421272.1 NZ_CP070008:c130759-130484 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T192653 NZ_CP092500:922418-922753 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGTCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT192653 WP_001387467.1 NZ_CP070008:c131043-130759 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT192653 NZ_CP092500:922170-922418 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880