Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 116366..116967 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP070008 | ||
| Organism | Escherichia coli strain FDAARGOS_1288 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | V0AJ64 |
| Locus tag | I6K18_RS26280 | Protein ID | WP_001216034.1 |
| Coordinates | 116587..116967 (+) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | U9YQH9 |
| Locus tag | I6K18_RS26275 | Protein ID | WP_001190712.1 |
| Coordinates | 116366..116587 (+) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| I6K18_RS26245 (111437) | 111437..111892 | - | 456 | Protein_127 | RepB family plasmid replication initiator protein | - |
| I6K18_RS26250 (112666) | 112666..113472 | - | 807 | WP_000016982.1 | site-specific integrase | - |
| I6K18_RS26255 (113473) | 113473..113778 | - | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | - |
| I6K18_RS26260 (113780) | 113780..113998 | - | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| I6K18_RS26270 (115251) | 115251..116183 | - | 933 | Protein_132 | type I restriction-modification system subunit M | - |
| I6K18_RS26275 (116366) | 116366..116587 | + | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| I6K18_RS26280 (116587) | 116587..116967 | + | 381 | WP_001216034.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| I6K18_RS26285 (116972) | 116972..117151 | + | 180 | WP_001513661.1 | hypothetical protein | - |
| I6K18_RS26290 (117179) | 117179..117457 | + | 279 | Protein_136 | pdcB | - |
| I6K18_RS26295 (117462) | 117462..117875 | + | 414 | Protein_137 | integrase core domain-containing protein | - |
| I6K18_RS26300 (117825) | 117825..118160 | - | 336 | WP_169329198.1 | type I deoxyribonuclease HsdR | - |
| I6K18_RS26305 (118370) | 118370..119350 | - | 981 | WP_000019407.1 | IS5-like element IS5 family transposase | - |
| I6K18_RS26310 (119594) | 119594..120997 | + | 1404 | WP_001373486.1 | S-methylmethionine permease | - |
| I6K18_RS26315 (120984) | 120984..121916 | + | 933 | WP_023143441.1 | homocysteine S-methyltransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | tet(B) / dfrA14 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 | - | 1..198318 | 198318 | |
| - | inside | IScluster/Tn | - | - | 110186..122747 | 12561 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13615.32 Da Isoelectric Point: 5.1514
>T192652 WP_001216034.1 NZ_CP070008:116587-116967 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE
Download Length: 381 bp
>T192652 NZ_CP092500:833619-834032 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA
Antitoxin
Download Length: 74 a.a. Molecular weight: 8133.10 Da Isoelectric Point: 4.7708
>AT192652 WP_001190712.1 NZ_CP070008:116366-116587 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT192652 NZ_CP092500:833372-833638 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AJ64 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJB6 |