Detailed information of TA system TA192643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 5170317..5171022
• Replicon: chromosome
• Accession: NZ_CP070007
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: I6K18_RS25100
• Protein ID: WP_000539521.1
• Coordinates: 5170317..5170703 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: I6K18_RS25105
• Protein ID: WP_001280945.1
• Coordinates: 5170693..5171022 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS25080 (5166321)	5166321..5166947	+	627	WP_001295292.1	glutathione S-transferase GstB	-
I6K18_RS25085 (5166944)	5166944..5168059	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
I6K18_RS25090 (5168170)	5168170..5168553	-	384	WP_000497137.1	biofilm formation regulator BssR	-
I6K18_RS25095 (5168766)	5168766..5170091	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
I6K18_RS25100 (5170317)	5170317..5170703	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K18_RS25105 (5170693)	5170693..5171022	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
I6K18_RS25110 (5171092)	5171092..5172420	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
I6K18_RS25115 (5172428)	5172428..5174776	-	2349	Protein_4951	cyclic diguanylate phosphodiesterase	-
I6K18_RS25120 (5174954)	5174954..5175865	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T192643 WP_000539521.1 NZ_CP070007:5170317-5170703 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T192643 NZ_CP092494:c41519-41244 [Citrobacter freundii]
ATGACACGCTACCGGACAGAATACACAAAACGGTTCGTCAAAGAGTTTAAGAAAATTGACACGGTACACCAGAGGGTAAT
TCAGAAGTACATTGAGGCGCACATCAACAACGCACTCAATCCGCGCTTGACGGGTAAAATCCTGAAAGGAGATAAAGCCG
AATTTATCCGCTGGCGTATCGGTGATTACCGTCTGATTGCCACTATTGAAGACGATAAATTGCTGGTCATTGCGTTAAGC
GTGGGACACCGCAAAGACGTGTACAAAAAGTTTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT192643 WP_001280945.1 NZ_CP070007:5170693-5171022 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT192643 NZ_CP092494:c41755-41531 [Citrobacter freundii]
ATGAACAAAAACATGACGAAAGCGATCCGGTTGAATGACGAAGATTTTAACCTGTTTGAGTCTTACGCCAACGCAAAAGG
GATAACCTTCAGCGAATTGTGCAAAAGCGCAGTACGGGAAAAAATCGAGGACGAATTAGATTTGCAATGTGCTAATGAAA
GCTACGCGGATTATCTCAGCGACAAAACGACAATCAGCCACGACGAACTGTTTAAATCTATGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6