Detailed information of TA system TA192641

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4525197..4525835
• Replicon: chromosome
• Accession: NZ_CP070007
• Organism: Escherichia coli strain FDAARGOS_1288

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: I6K18_RS21795
• Protein ID: WP_000813797.1
• Coordinates: 4525659..4525835 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: I6K18_RS21790
• Protein ID: WP_076611057.1
• Coordinates: 4525197..4525613 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K18_RS21770 (4520349)	4520349..4521290	-	942	WP_001251335.1	ABC transporter permease	-
I6K18_RS21775 (4521291)	4521291..4522304	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
I6K18_RS21780 (4522322)	4522322..4523467	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
I6K18_RS21785 (4523712)	4523712..4525118	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
I6K18_RS21790 (4525197)	4525197..4525613	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
I6K18_RS21795 (4525659)	4525659..4525835	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
I6K18_RS21800 (4526057)	4526057..4526287	+	231	WP_000494244.1	YncJ family protein	-
I6K18_RS21805 (4526379)	4526379..4528340	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
I6K18_RS21810 (4528413)	4528413..4528949	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
I6K18_RS21815 (4529041)	4529041..4530213	+	1173	WP_001236223.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T192641 WP_000813797.1 NZ_CP070007:c4525835-4525659 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T192641 NZ_CP092493:c4795429-4795070 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACTTGCTTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTTTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCAGGGGTTC
TGGAGGTCGTTGAGGATTACCACGGTAGCGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAATCCGTTTCCGGGAAGGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCATATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT192641 WP_076611057.1 NZ_CP070007:c4525613-4525197 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT192641 NZ_CP092493:c4795080-4794763 [Citrobacter freundii]
ATGTCATATGAAGTGGAAGCCAGTAGCGGTAATGTTTACGTCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAACTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGCTACTGGGAA
TGACGCAGCCTAAACTTTCCAACCTGTTACGCGGTCAGTTTCGAGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGACTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A829J715