Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2750016..2750670 Replicon chromosome
Accession NZ_CP070007
Organism Escherichia coli strain FDAARGOS_1288

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag I6K18_RS13270 Protein ID WP_000244769.1
Coordinates 2750263..2750670 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K18_RS13265 Protein ID WP_000354046.1
Coordinates 2750016..2750282 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K18_RS13245 (2746104) 2746104..2747537 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
I6K18_RS13250 (2747582) 2747582..2747893 + 312 WP_059326874.1 N(4)-acetylcytidine aminohydrolase -
I6K18_RS13255 (2748057) 2748057..2748716 + 660 WP_000250274.1 hemolysin III family protein -
I6K18_RS13260 (2748793) 2748793..2749773 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
I6K18_RS13265 (2750016) 2750016..2750282 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K18_RS13270 (2750263) 2750263..2750670 + 408 WP_000244769.1 protein YgfX Toxin
I6K18_RS13275 (2750710) 2750710..2751231 - 522 WP_001055874.1 flavodoxin FldB -
I6K18_RS13280 (2751343) 2751343..2752239 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
I6K18_RS13285 (2752264) 2752264..2752974 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K18_RS13290 (2752980) 2752980..2754713 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T192630 WP_000244769.1 NZ_CP070007:2750263-2750670 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192630 NZ_CP092493:c432207-431890 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192630 WP_000354046.1 NZ_CP070007:2750016-2750282 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192630 NZ_CP092493:c431837-431541 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References