Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1328176..1328673 Replicon chromosome
Accession NZ_CP070007
Organism Escherichia coli strain FDAARGOS_1288

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6K18_RS06525 Protein ID WP_001173343.1
Coordinates 1328176..1328349 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6K18_RS06530 Protein ID WP_000398619.1
Coordinates 1328377..1328673 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K18_RS06515 (1324847) 1324847..1326304 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
I6K18_RS06520 (1326541) 1326541..1328058 + 1518 WP_000003806.1 lysine--tRNA ligase -
I6K18_RS06525 (1328176) 1328176..1328349 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6K18_RS06530 (1328377) 1328377..1328673 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6K18_RS06535 (1328902) 1328902..1329174 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
I6K18_RS06540 (1329186) 1329186..1329416 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
I6K18_RS06545 (1329597) 1329597..1331228 + 1632 WP_001216477.1 sensor histidine kinase -
I6K18_RS06550 (1331225) 1331225..1331944 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T192624 WP_001173343.1 NZ_CP070007:c1328349-1328176 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T192624 NZ_CP092488:2932362-2932769 [Mycobacterium paraterrae]
GTGATCGTCGATACGTCAGCGATCATCGCGATCCTTCGCCAGGAAGACGACGCCGGGATCTTCGCTCAGGTCATCGCGCA
CGCTGACACCCGAAGGCTCTCTGCGGCAAGCTATCTCGAATGCGGGATCGTTCTCGATTTCCAGCGAGATCCGATCGTCA
GCCGAGCTCTCGACGCACTCGTCGAGGAGGCAGGTTTCGTGATTGAGCCGGTGACGGAACACCAGGCCCGCCTGGCGCGG
CAGGCCTACGCAGATTTCGGCAAAGGACGCCACGTGGCCGGGCTGAACTTCGGCGACTGCCTCGCATACGCCCTCGCGCT
CGACCGACGGGAACCCCTGTTGTGGAAGGGCGACGACTTCGGTCACACGGGTGTCGAGTCCGCTCTCGTAAAAGAAGCCG
GGTCCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT192624 WP_000398619.1 NZ_CP070007:c1328673-1328377 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT192624 NZ_CP092488:2932120-2932365 [Mycobacterium paraterrae]
ATGGCATTGAACATTAAAGATGTGTCGGTGCACGAAGTGGTCAAGCAGATCGCGAAGCTCACCGGCGAGTCCCAGGCGCA
GGCTGTTGCGACTGCGGTCAGGGAACGCCTCGCGCGACTGCAGGGCGACGACCTTGCGGCCCGCCTCCTTGCCATCGGTC
ATAAGACCGCGAGTCGGATGAGCCCCGAGACCAAAATGCTGGATCACGACACGCTGCTCTACGACGGGCGCGGGCTGCCC
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References