Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 396388..397006 Replicon chromosome
Accession NZ_CP070007
Organism Escherichia coli strain FDAARGOS_1288

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K18_RS02020 Protein ID WP_001291435.1
Coordinates 396788..397006 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K18_RS02015 Protein ID WP_000344800.1
Coordinates 396388..396762 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K18_RS02005 (391477) 391477..392670 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K18_RS02010 (392693) 392693..395842 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
I6K18_RS02015 (396388) 396388..396762 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K18_RS02020 (396788) 396788..397006 + 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K18_RS02025 (397178) 397178..397729 + 552 WP_000102559.1 maltose O-acetyltransferase -
I6K18_RS02030 (397845) 397845..398315 + 471 WP_000136192.1 YlaC family protein -
I6K18_RS02035 (398479) 398479..400029 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K18_RS02040 (400071) 400071..400424 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K18_RS02050 (400803) 400803..401114 + 312 WP_000409911.1 MGMT family protein -
I6K18_RS02055 (401145) 401145..401717 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192615 WP_001291435.1 NZ_CP070007:396788-397006 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192615 NZ_CP092478:873600-873806 [Staphylococcus haemolyticus]
ATGAACTTTAGTGAAATTAGAATTAGTGAATTAAATACTGAAGAGCAAGCTAATTCACTCCAACATAGACTTGAGAAGAT
GATAGGTGTAGATGAAGTAATTGTAGAACTAAATGACCAATTAGTTAAAATTTCTTATGAAACACCTACGAATTTAAATA
GTATTGAAAAAGAAATTTATGATGATGGTTATAAGGTATTAGGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192615 WP_000344800.1 NZ_CP070007:396388-396762 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192615 NZ_CP092478:873295-873588 [Staphylococcus haemolyticus]
TTGAATAATCATGAAAATGCACATCATTCCGATCAAATTAAAATGAATTTAAAATCAAGACTTAATCGTATTGAAGGGCA
AGTTAGAGCGATTAATCGCATGATTGAAGAAGATGTATACTGTGATGACGTGCTAACTCAAATTAGAGCTACAAGATCAG
CTTTAAATAGTGTGGCCACTAAGTTGCTAGATCATCATATGAAAAGCTGTATCATGGATAAAGTGAATAACGGCGCTCAA
GAAGAAGCGATGGAAGAATTGTTAGTGACTTTTCAAAAATTAATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References