Detailed information of TA system TA192613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 28054..28601
• Replicon: plasmid unnamed2
• Accession: NZ_CP069998
• Organism: Escherichia coli strain FDAARGOS_1300

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: I6K30_RS25745
• Protein ID: WP_001384452.1
• Coordinates: 28054..28332 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: I6K30_RS25750
• Protein ID: WP_000079941.1
• Coordinates: 28332..28601 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K30_RS25715 (23484)	23484..23744	+	261	WP_000343091.1	hypothetical protein	-
I6K30_RS25720 (23741)	23741..24331	+	591	WP_001350923.1	hypothetical protein	-
I6K30_RS25725 (24393)	24393..24608	-	216	Protein_24	DUF6404 family protein	-
I6K30_RS25730 (24737)	24737..25072	+	336	WP_001080729.1	colicin 1A immunity protein	-
I6K30_RS25735 (25094)	25094..26974	-	1881	WP_001283335.1	colicin Ia central receptor-binding domain-containing protein	-
I6K30_RS25740 (27160)	27160..28008	-	849	WP_001057989.1	3'-5' exonuclease	-
I6K30_RS25745 (28054)	28054..28332	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
I6K30_RS25750 (28332)	28332..28601	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
I6K30_RS25755 (29517)	29517..30374	-	858	WP_000130940.1	incFII family plasmid replication initiator RepA	-
I6K30_RS27300 (30367)	30367..30414	-	48	WP_077822033.1	hypothetical protein	-
I6K30_RS27305 (30405)	30405..30641	+	237	WP_223368648.1	replication protein RepA	-
I6K30_RS25765 (30667)	30667..30927	-	261	WP_000083818.1	replication regulatory protein RepA	-
I6K30_RS25770 (31017)	31017..31373	-	357	Protein_34	thermonuclease family protein	-
I6K30_RS25775 (31616)	31616..31828	-	213	WP_001311693.1	hypothetical protein	-
I6K30_RS25780 (31966)	31966..32526	-	561	WP_000139359.1	fertility inhibition protein FinO	-
I6K30_RS25785 (32581)	32581..33327	-	747	WP_000205759.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..132040	132040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T192613 WP_001384452.1 NZ_CP069998:c28332-28054 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T192613 NZ_CP092475:1304808-1304984 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGGTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT192613 WP_000079941.1 NZ_CP069998:c28601-28332 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT192613 NZ_CP092475:c1305146-1304972 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: A0A829CL36

Antitoxin

• AlphaFold DB: A0A629TXC5