Detailed information of TA system TA192610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4764912..4766500
• Replicon: chromosome
• Accession: NZ_CP069996
• Organism: Escherichia coli strain FDAARGOS_1300

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6K30_RS23230
• Protein ID: WP_001125475.1
• Coordinates: 4765178..4766500 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K30_RS23225
• Protein ID: WP_001332145.1
• Coordinates: 4764912..4765178 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K30_RS23210 (4761280)	4761280..4762731	+	1452	WP_001513806.1	tagaturonate reductase	-
I6K30_RS23215 (4762928)	4762928..4763842	+	915	WP_001306069.1	bestrophin family protein	-
I6K30_RS23220 (4763846)	4763846..4764604	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6K30_RS23225 (4764912)	4764912..4765178	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K30_RS23230 (4765178)	4765178..4766500	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K30_RS23235 (4766675)	4766675..4767046	-	372	WP_000795853.1	hypothetical protein	-
I6K30_RS23240 (4767092)	4767092..4767559	-	468	WP_000218697.1	hypothetical protein	-
I6K30_RS23245 (4767671)	4767671..4768093	-	423	WP_000089313.1	hypothetical protein	-
I6K30_RS23250 (4768193)	4768193..4768639	-	447	WP_000527682.1	helix-turn-helix transcriptional regulator	-
I6K30_RS23255 (4768811)	4768811..4769863	+	1053	WP_000568107.1	phage/plasmid replication protein, II/X family	-
I6K30_RS23260 (4769875)	4769875..4770192	+	318	WP_000073835.1	DNA-binding protein	-
I6K30_RS23265 (4770195)	4770195..4770446	+	252	WP_000381239.1	membrane protein	-
I6K30_RS23270 (4770481)	4770481..4770714	+	234	WP_000473544.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4763846..4787058	23212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T192610 WP_001125475.1 NZ_CP069996:4765178-4766500 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192610 NZ_CP092475:782708-782884 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT192610 WP_001332145.1 NZ_CP069996:4764912-4765178 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT192610 NZ_CP092475:c783046-782872 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8