Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4264323..4265154 Replicon chromosome
Accession NZ_CP069996
Organism Escherichia coli strain FDAARGOS_1300

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K30_RS20650 Protein ID WP_000854814.1
Coordinates 4264323..4264697 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag I6K30_RS20655 Protein ID WP_001285584.1
Coordinates 4264786..4265154 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K30_RS20610 (4259719) 4259719..4260885 + 1167 WP_001513842.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
I6K30_RS20615 (4261004) 4261004..4261477 + 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
I6K30_RS20620 (4261675) 4261675..4262733 + 1059 WP_001200888.1 FUSC family protein -
I6K30_RS20625 (4262905) 4262905..4263234 + 330 WP_000450409.1 DUF496 family protein -
I6K30_RS20630 (4263335) 4263335..4263469 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
I6K30_RS20635 (4263589) 4263589..4263717 + 129 Protein_4071 transposase domain-containing protein -
I6K30_RS20640 (4264006) 4264006..4264086 - 81 Protein_4072 hypothetical protein -
I6K30_RS20645 (4264132) 4264132..4264326 - 195 WP_000988600.1 DUF5983 family protein -
I6K30_RS20650 (4264323) 4264323..4264697 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K30_RS20655 (4264786) 4264786..4265154 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K30_RS20660 (4265228) 4265228..4265449 - 222 WP_000692323.1 DUF987 domain-containing protein -
I6K30_RS20665 (4265512) 4265512..4265988 - 477 WP_001186773.1 RadC family protein -
I6K30_RS20670 (4266004) 4266004..4266483 - 480 WP_000860076.1 antirestriction protein -
I6K30_RS20675 (4266565) 4266565..4267383 - 819 WP_001234629.1 DUF932 domain-containing protein -
I6K30_RS20680 (4267483) 4267483..4267716 - 234 WP_001119729.1 DUF905 family protein -
I6K30_RS20685 (4267795) 4267795..4268250 - 456 WP_000581504.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T192601 WP_000854814.1 NZ_CP069996:c4264697-4264323 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T192601 NZ_CP092470:c1262772-1262518 [Bifidobacterium pseudocatenulatum]
ATAATGCTGCGTTGGACTGATGACGCGTGGAACGATTACCTGTATTGGCAGGTTCAGGATAAGAAAACCCTCAAGCGGGT
CAACGCGCTGATACGTGATGCGATGCGTTCTCCATTCGCGGGTCTCGGTAAGCCAGAGCCGCTCAAATGGGAACTGCAGG
GTGCGTGGAGCCGCCGCATCGATTCCGCAAACCGTCTGGTATACATGATTGAGGATGGCGACTTGTGCATTCTGTCCGTC
CGTGACCACTATTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT192601 WP_001285584.1 NZ_CP069996:c4265154-4264786 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT192601 NZ_CP092470:c1263032-1262772 [Bifidobacterium pseudocatenulatum]
ATGGTCACGGCGGTCGCGTACACCAATTTCCGCAAGGATCTGAAATCATATCTACGTCGCGTGAACGATGACGCGGATAT
GCTTCTGATTACCAACAAGGATCCAGGGGACAACGTGGTGGTGATGAGCGCCGATGATTACGATTCGCTTATGGAGACAT
TGCGCGTTTACCAGAATCCCGGATTGTTCGATAAAGTCCGACGTGGCATGGCGCAGGTACGCTCAGGTTCCACCGATTCA
CATGATCTGGTCGGAGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References