Detailed information of TA system TA192598

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3237004..3237658
• Replicon: chromosome
• Accession: NZ_CP069996
• Organism: Escherichia coli strain FDAARGOS_1300

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: I6K30_RS15780
• Protein ID: WP_000244781.1
• Coordinates: 3237251..3237658 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: I6K30_RS15775
• Protein ID: WP_016239318.1
• Coordinates: 3237004..3237270 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K30_RS15755 (3233092)	3233092..3234525	-	1434	WP_001350137.1	6-phospho-beta-glucosidase BglA	-
I6K30_RS15760 (3234570)	3234570..3234881	+	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
I6K30_RS15765 (3235045)	3235045..3235704	+	660	WP_000250274.1	hemolysin III family protein	-
I6K30_RS15770 (3235781)	3235781..3236761	-	981	WP_000886078.1	tRNA-modifying protein YgfZ	-
I6K30_RS15775 (3237004)	3237004..3237270	+	267	WP_016239318.1	FAD assembly factor SdhE	Antitoxin
I6K30_RS15780 (3237251)	3237251..3237658	+	408	WP_000244781.1	protein YgfX	Toxin
I6K30_RS15785 (3237698)	3237698..3238219	-	522	WP_001055874.1	flavodoxin FldB	-
I6K30_RS15790 (3238331)	3238331..3239227	+	897	WP_000806987.1	site-specific tyrosine recombinase XerD	-
I6K30_RS15795 (3239252)	3239252..3239962	+	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
I6K30_RS15800 (3239968)	3239968..3241701	+	1734	WP_000813190.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T192598 WP_000244781.1 NZ_CP069996:3237251-3237658 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T192598 NZ_CP092466:c4936455-4936144 [Citrobacter portucalensis]
ATGCTATTCATAGAAACGGAAATCTTCACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGATTACAGAT
ATTCCTTGCGATTCAGCCTGATTGTGGCGATCTGATACAAGAAACGGGCGGACTGAGAAAGGTTCGCTGGAGATCGCGAG
GGAAAGGCAAACGTAGCGGTGTGAGGATTATTTACTTTCATCAAATCAGAAAATCACAAATTCGATTGTTGCTGATTTAT
CAGAAAGGGATCAAGGATGATCTGACTCCACAAGAAAAAGTGCTGCTGCGTATGTTGAATGAGAGGTGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10573.20 Da
• Isoelectric Point: 5.1599

>AT192598 WP_016239318.1 NZ_CP069996:3237004-3237270 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDILSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT192598 NZ_CP092466:c4936143-4935856 [Citrobacter portucalensis]
ATGGATAAGGCACTATTTGAGCGATTAACTCAAAGTATGGTTCAGATGAATGAGATCGCTGAAGGAACGCGACAACCTTC
CCGTACCTTTCATATTGACGCCATGAAAATAAAGGAAATTCGCCAGTCTTCAGGATTATCACAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTGGATACGTTGCGAAATTGGGAGCAAGGGCGTCGTTCACCGACCGGTCCAGCAAAAGCGTTGTTA
CGTGCTATTGCCAATGATCCAAAACATGTCATTCAGGCACTAAGATAA

Structures

Toxin

• AlphaFold DB: S1PAM6