Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2305376..2306177 Replicon chromosome
Accession NZ_CP069996
Organism Escherichia coli strain FDAARGOS_1300

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0E0XS49
Locus tag I6K30_RS11230 Protein ID WP_001094437.1
Coordinates 2305376..2305753 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0E0XTQ1
Locus tag I6K30_RS11235 Protein ID WP_001390338.1
Coordinates 2305800..2306177 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K30_RS11200 (2300741) 2300741..2301559 + 819 WP_000779409.1 lipoprotein NlpA -
I6K30_RS11205 (2301563) 2301563..2302486 - 924 WP_001351210.1 carboxylate/amino acid/amine transporter -
I6K30_RS11210 (2302783) 2302783..2303151 - 369 WP_000509809.1 hypothetical protein -
I6K30_RS11215 (2303744) 2303744..2304589 - 846 WP_001274561.1 DUF4942 domain-containing protein -
I6K30_RS11220 (2304674) 2304674..2304871 - 198 WP_000772032.1 DUF957 domain-containing protein -
I6K30_RS11225 (2304891) 2304891..2305379 - 489 WP_000761716.1 DUF5983 family protein -
I6K30_RS11230 (2305376) 2305376..2305753 - 378 WP_001094437.1 TA system toxin CbtA family protein Toxin
I6K30_RS11235 (2305800) 2305800..2306177 - 378 WP_001390338.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
I6K30_RS11240 (2306340) 2306340..2306561 - 222 WP_000692345.1 DUF987 domain-containing protein -
I6K30_RS11245 (2306624) 2306624..2307100 - 477 WP_001535682.1 RadC family protein -
I6K30_RS11250 (2307116) 2307116..2307589 - 474 WP_000855064.1 antirestriction protein -
I6K30_RS11255 (2307931) 2307931..2308749 - 819 WP_001535681.1 DUF932 domain-containing protein -
I6K30_RS11260 (2308867) 2308867..2309062 - 196 Protein_2211 DUF905 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B - 2302783..2349138 46355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13986.90 Da        Isoelectric Point: 7.3223

>T192590 WP_001094437.1 NZ_CP069996:c2305753-2305376 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Download         Length: 378 bp

>T192590 NZ_CP092466:c2611462-2611184 [Citrobacter portucalensis]
ATGATCATGAACTTCAGGCACAAGGGATTGCGCGATTTGTTTCTTCATGGACGAACATCAGGCGTTATGGCGAAACATGT
CCGACGATTACGACACAGACTTGCCGTTATTGATGCGGCAAGCAAGGTTACAGATATCAATATGCCCGGTTATAAGCTAC
ATCCTCTGGTGGGGGAACGCGACGGCATATGGGCAATTTCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGATTACGAGGACTATCATTGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13745.44 Da        Isoelectric Point: 5.0463

>AT192590 WP_001390338.1 NZ_CP069996:c2306177-2305800 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYAYLAVYPTPAEPAITV

Download         Length: 378 bp

>AT192590 NZ_CP092466:c2611184-2610900 [Citrobacter portucalensis]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGAAGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAGATTGCACCGTCAACGGCAAGTCGACTATTAACCGGGAAAGCGGCTCTGACGCCTGAGATGGCGA
TTAAACTCTCCGTAGTGATTGGCAGTTCACCCGAAATGTGGTTAAACCTGCAAAATACCTGGAGTCTGGCAGAGGCCCAA
AAAAGCGTGGATATTTCACGTCTGCGTAAGCTGGCGGTACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XS49

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XTQ1

References