Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 704653..705271 Replicon chromosome
Accession NZ_CP069996
Organism Escherichia coli strain FDAARGOS_1300

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K30_RS03455 Protein ID WP_001291435.1
Coordinates 705053..705271 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K30_RS03450 Protein ID WP_000344800.1
Coordinates 704653..705027 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K30_RS03440 (699743) 699743..700936 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K30_RS03445 (700959) 700959..704108 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K30_RS03450 (704653) 704653..705027 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K30_RS03455 (705053) 705053..705271 + 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K30_RS03460 (705445) 705445..705996 + 552 WP_000102543.1 maltose O-acetyltransferase -
I6K30_RS03465 (706112) 706112..706582 + 471 WP_000136192.1 YlaC family protein -
I6K30_RS03470 (706746) 706746..708296 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K30_RS03475 (708338) 708338..708691 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6K30_RS03485 (709070) 709070..709381 + 312 WP_000409908.1 MGMT family protein -
I6K30_RS03490 (709412) 709412..709984 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192578 WP_001291435.1 NZ_CP069996:705053-705271 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192578 NZ_CP092460:c4034074-4033664 [Dickeya fangzhongdai]
GTGAACAGAACGTATATGCTCGATACCCGTTTCTGCGCGTTCATCCTGCGCGAGCAGCCGGAAGCCGTGCTCGGGCGGCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGATCTCCGCCGTCACCTGGGCCGAACTGTCACAGGCCGCACGGGAGG
ACAGTCCCGCCACGCAGGCTCTGGCCGATGCGTTCTGCGCCAGTCTCGATGCCGTGCTGCCCTGGGATCGCGCCGCGGTG
GATGCGACCACGGAAATTAAGGCAGCCCTGGCGGCGACCGGAACCCCGCTCGGCCCGAACGATGCGGCGATTGCCGGGCA
TGCCATCGCCGCCGGCGCGGTGCTGGTGACCAGGAATGGAGAAGAGTTTGAGCGGGTGCCGGGTCTCATACTGCAAGACT
GGGCCGGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192578 WP_000344800.1 NZ_CP069996:704653-705027 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192578 NZ_CP092460:c4034301-4034071 [Dickeya fangzhongdai]
ATGAGAACCGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGTCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACTGCATCATCCTGCGTCCCGTCCGCCCGGCCTGGAGCTCATTCGCCCTGATGG
ACAGGGCCGGGCCTGAGTTTATGGCGGAGCGTGAAGTCGTTATAAGTGATAACGGGCGGGGTGAACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References