Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 120934..121670 Replicon plasmid unnamed
Accession NZ_CP069995
Organism Klebsiella pneumoniae strain FDAARGOS_1321

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag I6K51_RS26070 Protein ID WP_003026803.1
Coordinates 121188..121670 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag I6K51_RS26065 Protein ID WP_003026799.1
Coordinates 120934..121200 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K51_RS26020 116996..117358 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
I6K51_RS26025 117408..117758 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
I6K51_RS26030 118116..118385 + 270 WP_004152102.1 hypothetical protein -
I6K51_RS26035 118373..118948 + 576 WP_004152103.1 hypothetical protein -
I6K51_RS26040 118979..119473 + 495 WP_004152104.1 DNA-binding protein -
I6K51_RS26045 119517..119885 + 369 WP_004152105.1 hypothetical protein -
I6K51_RS26050 119919..120122 + 204 WP_004152106.1 hemolysin expression modulator Hha -
I6K51_RS26055 120171..120428 + 258 WP_004152107.1 hypothetical protein -
I6K51_RS26060 120504..120758 + 255 WP_004152108.1 hypothetical protein -
I6K51_RS26065 120934..121200 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
I6K51_RS26070 121188..121670 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
I6K51_RS26075 121882..123228 + 1347 WP_020314316.1 ISNCY family transposase -
I6K51_RS26080 123729..124983 - 1255 Protein_122 IS3 family transposase -
I6K51_RS26085 125071..126033 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / blaSHV-1 - 1..181659 181659


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T192576 WP_003026803.1 NZ_CP069995:121188-121670 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T192576 NZ_CP092460:1957062-1957376 [Dickeya fangzhongdai]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGCCATTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAGTTGAATCGTCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTACCCGGCAGGCCGCCGACCGGAGCGCCTTGTCC
CTGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTTCAGGCCCTGGGG
GCGGTGTTTTGTGATGCGTCGCAGTACCGTAATCAGGTAAAGGCCGCAATAGATTTCCTCATCGACGGCATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT192576 WP_003026799.1 NZ_CP069995:120934-121200 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT192576 NZ_CP092460:1956823-1957059 [Dickeya fangzhongdai]
ATGACCGTGAAACAACGCAACACGCAGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGGTCCGGGCGCGTGAAGCTGG
CATTAACCTGAGTGCCACCCTGTCAACCGCACTGGATGCGGAGCTTCGCCGTTATGAAGCGAACAAATGGCAGGAAGAGA
ACAGGGAAGCCATTGACGCATTAAACCGTTTTCATGATGAACACGGCTGTTTCAGCGATGAATACAGGACCTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References