Detailed information of TA system TA192558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 124447..125183
• Replicon: plasmid unnamed
• Accession: NZ_CP069993
• Organism: Klebsiella pneumoniae strain FDAARGOS_1329

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: I6K59_RS26785
• Protein ID: WP_004187044.1
• Coordinates: 124447..124929 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I6K59_RS26790
• Protein ID: WP_003026799.1
• Coordinates: 124917..125183 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K59_RS26760	120978..121914	-	937	Protein_131	ISNCY family transposase	-
I6K59_RS26765	122024..122194	+	171	Protein_132	transposase	-
I6K59_RS26770	122192..122299	+	108	Protein_133	IS3 family transposase	-
I6K59_RS26775	122447..122845	-	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
I6K59_RS26780	122894..124240	-	1347	WP_077254728.1	ISNCY family transposase	-
I6K59_RS26785	124447..124929	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
I6K59_RS26790	124917..125183	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I6K59_RS26795	126295..127104	-	810	WP_023329017.1	N-formylglutamate deformylase	-
I6K59_RS26800	127097..128305	-	1209	WP_032439650.1	imidazolonepropionase	-
I6K59_RS26805	128317..129711	-	1395	WP_032439652.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..178126	178126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T192558 WP_004187044.1 NZ_CP069993:c124929-124447 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T192558 NZ_CP092452:3591365-3591643 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT192558 WP_003026799.1 NZ_CP069993:c125183-124917 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT192558 NZ_CP092452:3591102-3591362 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66