Detailed information of TA system TA192538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 124084..124619
• Replicon: plasmid unnamed
• Accession: NZ_CP069991
• Organism: Escherichia coli strain FDAARGOS_1304

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0M1UJC2
• Locus tag: I6K34_RS25775
• Protein ID: WP_001270422.1
• Coordinates: 124332..124619 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: I6K34_RS25770
• Protein ID: WP_001132895.1
• Coordinates: 124084..124335 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K34_RS25740 (119143)	119143..119454	-	312	WP_001309256.1	hypothetical protein	-
I6K34_RS25745 (119451)	119451..119979	-	529	Protein_143	DUF1281 domain-containing protein	-
I6K34_RS26230 (119981)	119981..120064	+	84	Protein_144	DUF4113 domain-containing protein	-
I6K34_RS25755 (120194)	120194..120970	-	777	Protein_145	transposase DNA-binding-containing protein	-
I6K34_RS25760 (121204)	121204..122166	-	963	WP_000756328.1	ParB family protein	-
I6K34_RS25765 (122163)	122163..123368	-	1206	WP_000817635.1	AAA family ATPase	-
I6K34_RS25770 (124084)	124084..124335	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6K34_RS25775 (124332)	124332..124619	+	288	WP_001270422.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K34_RS25780 (124621)	124621..125032	+	412	Protein_150	DDE-type integrase/transposase/recombinase	-
I6K34_RS25785 (125676)	125676..126653	+	978	WP_001309253.1	RepB family plasmid replication initiator protein	-
I6K34_RS25790 (126896)	126896..127636	-	741	WP_001066947.1	tyrosine-type recombinase/integrase	-
I6K34_RS25795 (127757)	127757..127945	-	189	WP_001332784.1	hypothetical protein	-
I6K34_RS25800 (128312)	128312..129481	+	1170	WP_001072358.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	senB	1..133493	133493
	-	flank	IS/Tn	-	-	120407..120547	140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11262.30 Da
• Isoelectric Point: 10.4703

>T192538 WP_001270422.1 NZ_CP069991:124332-124619 [Escherichia coli]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T192538 NZ_CP092449:c3586087-3585689 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT192538 WP_001132895.1 NZ_CP069991:124084-124335 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT192538 NZ_CP092449:c3586383-3586090 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0M1UJC2

Antitoxin

• AlphaFold DB: L4J0V6