Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4754838..4755492 Replicon chromosome
Accession NZ_CP069990
Organism Escherichia coli strain FDAARGOS_1304

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag I6K34_RS23120 Protein ID WP_000244781.1
Coordinates 4755085..4755492 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6K34_RS23115 Protein ID WP_000354046.1
Coordinates 4754838..4755104 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K34_RS23095 (4750926) 4750926..4752359 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
I6K34_RS23100 (4752404) 4752404..4752715 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6K34_RS23105 (4752879) 4752879..4753538 + 660 WP_000250274.1 hemolysin III family protein -
I6K34_RS23110 (4753615) 4753615..4754595 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
I6K34_RS23115 (4754838) 4754838..4755104 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6K34_RS23120 (4755085) 4755085..4755492 + 408 WP_000244781.1 protein YgfX Toxin
I6K34_RS23125 (4755532) 4755532..4756053 - 522 WP_001055874.1 flavodoxin FldB -
I6K34_RS23130 (4756165) 4756165..4757061 + 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
I6K34_RS23135 (4757086) 4757086..4757796 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6K34_RS23140 (4757802) 4757802..4759535 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T192529 WP_000244781.1 NZ_CP069990:4755085-4755492 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T192529 NZ_CP092449:1783629-1783880 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT192529 WP_000354046.1 NZ_CP069990:4754838-4755104 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT192529 NZ_CP092449:1783877-1784131 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References