Detailed information of TA system TA192523

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2962062..2962896
• Replicon: chromosome
• Accession: NZ_CP069990
• Organism: Escherichia coli strain FDAARGOS_1304

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: I6K34_RS14430
• Protein ID: WP_000854770.1
• Coordinates: 2962062..2962439 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6K34_RS14435
• Protein ID: WP_001332042.1
• Coordinates: 2962528..2962896 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K34_RS14410 (2960586)	2960586..2960762	-	177	Protein_2847	helix-turn-helix domain-containing protein	-
I6K34_RS14415 (2961129)	2961129..2961278	-	150	Protein_2848	hypothetical protein	-
I6K34_RS14420 (2961384)	2961384..2961560	-	177	WP_000839286.1	DUF957 domain-containing protein	-
I6K34_RS14425 (2961577)	2961577..2962065	-	489	WP_000761690.1	DUF5983 family protein	-
I6K34_RS14430 (2962062)	2962062..2962439	-	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
I6K34_RS14435 (2962528)	2962528..2962896	-	369	WP_001332042.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6K34_RS14440 (2963059)	2963059..2963280	-	222	WP_000692323.1	DUF987 domain-containing protein	-
I6K34_RS14445 (2963343)	2963343..2963819	-	477	WP_001557203.1	RadC family protein	-
I6K34_RS14450 (2963835)	2963835..2964299	-	465	WP_000855061.1	antirestriction protein	-
I6K34_RS14455 (2964641)	2964641..2965459	-	819	WP_001234738.1	DUF932 domain-containing protein	-
I6K34_RS14460 (2965577)	2965577..2965772	-	196	Protein_2857	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2950140..2986673	36533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T192523 WP_000854770.1 NZ_CP069990:c2962439-2962062 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T192523 NZ_CP092447:c2355878-2355612 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGTTATTATAGTAGACGTATCAATCACGAACATAGATTGGTCTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13677.38 Da
• Isoelectric Point: 6.5931

>AT192523 WP_001332042.1 NZ_CP069990:c2962896-2962528 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT192523 NZ_CP092447:c2356129-2355878 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACCTCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGAAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9