Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2892112..2892688 Replicon chromosome
Accession NZ_CP069990
Organism Escherichia coli strain FDAARGOS_1304

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag I6K34_RS14085 Protein ID WP_001297643.1
Coordinates 2892401..2892688 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag I6K34_RS14080 Protein ID WP_000063156.1
Coordinates 2892112..2892414 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K34_RS14065 (2888708) 2888708..2890858 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
I6K34_RS14070 (2890908) 2890908..2891111 + 204 WP_000467859.1 YbdD/YjiX family protein -
I6K34_RS14075 (2891122) 2891122..2892078 + 957 WP_001297640.1 GTPase -
I6K34_RS14080 (2892112) 2892112..2892414 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
I6K34_RS14085 (2892401) 2892401..2892688 - 288 WP_001297643.1 BrnT family toxin Toxin
I6K34_RS14090 (2892887) 2892887..2893584 - 698 Protein_2781 IS1 family transposase -
I6K34_RS14095 (2893641) 2893641..2893736 + 96 Protein_2782 dienelactone hydrolase family protein -
I6K34_RS14100 (2893882) 2893882..2895114 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
I6K34_RS14105 (2895155) 2895155..2896435 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T192522 WP_001297643.1 NZ_CP069990:c2892688-2892401 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T192522 NZ_CP092447:c2032316-2032107 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT192522 WP_000063156.1 NZ_CP069990:c2892414-2892112 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT192522 NZ_CP092447:c2032621-2032328 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References