Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2353339..2353957 Replicon chromosome
Accession NZ_CP069990
Organism Escherichia coli strain FDAARGOS_1304

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6K34_RS11540 Protein ID WP_001291435.1
Coordinates 2353739..2353957 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6K34_RS11535 Protein ID WP_000344800.1
Coordinates 2353339..2353713 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K34_RS11525 (2348429) 2348429..2349622 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6K34_RS11530 (2349645) 2349645..2352794 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
I6K34_RS11535 (2353339) 2353339..2353713 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6K34_RS11540 (2353739) 2353739..2353957 + 219 WP_001291435.1 HHA domain-containing protein Toxin
I6K34_RS11545 (2354131) 2354131..2354682 + 552 WP_000102543.1 maltose O-acetyltransferase -
I6K34_RS11550 (2354798) 2354798..2355268 + 471 WP_000136192.1 YlaC family protein -
I6K34_RS11555 (2355432) 2355432..2356982 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6K34_RS11560 (2357024) 2357024..2357377 - 354 WP_000878145.1 DUF1428 family protein -
I6K34_RS11570 (2357756) 2357756..2358067 + 312 WP_000409908.1 MGMT family protein -
I6K34_RS11575 (2358098) 2358098..2358670 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T192518 WP_001291435.1 NZ_CP069990:2353739-2353957 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T192518 NZ_CP092447:c816557-816369 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATATTCGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT192518 WP_000344800.1 NZ_CP069990:2353339-2353713 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT192518 NZ_CP092447:c816846-816586 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References