Detailed information of TA system TA192515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1288238..1289826
• Replicon: chromosome
• Accession: NZ_CP069990
• Organism: Escherichia coli strain FDAARGOS_1304

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: I6K34_RS06270
• Protein ID: WP_001125475.1
• Coordinates: 1288504..1289826 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K34_RS06265
• Protein ID: WP_001332145.1
• Coordinates: 1288238..1288504 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K34_RS06250 (1284606)	1284606..1286057	+	1452	WP_000854637.1	tagaturonate reductase	-
I6K34_RS06255 (1286254)	1286254..1287168	+	915	WP_001306069.1	bestrophin family protein	-
I6K34_RS06260 (1287172)	1287172..1287930	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
I6K34_RS06265 (1288238)	1288238..1288504	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6K34_RS06270 (1288504)	1288504..1289826	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6K34_RS06275 (1290085)	1290085..1290342	-	258	WP_001210030.1	hypothetical protein	-
I6K34_RS06280 (1290362)	1290362..1290967	+	606	Protein_1242	SDR family oxidoreductase	-
I6K34_RS26035 (1291211)	1291211..1291318	-	108	Protein_1243	hypothetical protein	-
I6K34_RS06285 (1291743)	1291743..1292306	+	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
I6K34_RS06290 (1292668)	1292668..1293378	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T192515 WP_001125475.1 NZ_CP069990:1288504-1289826 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T192515 NZ_CP092442:4708624-4708935 [Citrobacter portucalensis]
GTGCATGTTATCAGCCGAAAACCTTTTAACGAGGCAATGCTCATGTATCCAAATCATCAGCTTGCTCTCGCTGATTTACT
GAATGTTTTGGAGAAAAAAACATTCATTCAGCCTGAAGAGATGAAGCAGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTAAGACTCATCTCCTACATAGATTTCAGGTCACATAAGATA
TTTGTCAAACATATCGTTTCCCATGCCGAATATGACAAACTGACAGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT192515 WP_001332145.1 NZ_CP069990:1288238-1288504 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT192515 NZ_CP092442:4708932-4709378 [Citrobacter portucalensis]
ATGAGAACGCATCGTCATATGGATGCAGCCAGCGCAAAAAAGATTGTTGATACCTTCAGCGATGCGGTAAAAACCATCCC
ACTGTTGGGGGAAGACCGAAATGACACTGAGTATCGCAGGGCACTTGCGCTAGTGGAGTATCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTAAATATGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACACCCTCTGGCGTTTCAGTATTGCGTACGCTCATGGACCAATACAGTCTCAAAGCAGCAGACCT
TGCCAACGAACTCGGTTCAAAATCGAACGTCAGCAACATCTTAAATGGTCGCAGAGCACTGACTGTAAGCCATATTAAAG
CGCTTGCAGAACGCTTTAATCTTCCGGCAGACGCATTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8