Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 54024..54549 Replicon plasmid unnamed2
Accession NZ_CP069982
Organism Escherichia coli strain FDAARGOS_1291

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag I6K21_RS24855 Protein ID WP_001159868.1
Coordinates 54244..54549 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6K21_RS24850 Protein ID WP_000813634.1
Coordinates 54024..54242 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K21_RS24825 (49159) 49159..49380 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
I6K21_RS24830 (49563) 49563..50495 + 933 Protein_64 type I restriction-modification system subunit M -
I6K21_RS24835 (50551) 50551..51248 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
I6K21_RS24840 (51385) 51385..52278 - 894 WP_001553853.1 S-4TM family putative pore-forming effector -
I6K21_RS24845 (52321) 52321..53316 - 996 WP_000246636.1 hypothetical protein -
I6K21_RS24850 (54024) 54024..54242 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6K21_RS24855 (54244) 54244..54549 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
I6K21_RS24860 (54550) 54550..55356 + 807 WP_000016982.1 site-specific integrase -
I6K21_RS24865 (56130) 56130..56885 + 756 WP_000852146.1 replication initiation protein RepE -
I6K21_RS24870 (57473) 57473..58639 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(B) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / dfrA14 - 1..116491 116491
- inside IScluster/Tn - - 42873..51248 8375


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T192498 WP_001159868.1 NZ_CP069982:54244-54549 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T192498 NZ_CP092437:8215-8574 [Rhizobium sp. K102]
ATGGTCCGCAGCAATGCTCCGAAGCGTGGCGACGTCTTTCTCGTCGACCTGAATCCAGTCGTCGGGAATGAAATGAAGGA
TGGACATCGATGCGTCGTGATCAGCCCTCGCGAGATCAATCTCGTGGGACTGTGCCTCACCGTCCCCATTACTACGGGAG
GCGTGTTTACTCGTAAGGCTGGACTGGCTGTGAACATCTCAGGCCATAAGACGACCGGGGTCGCTCTCTGCAATCAAGTC
AGAGCTCTCGACATCATGGAGCGCGTAAGACTTAAAACCGCTCGGCTTGTAGATACGTTGGACACCCGCACGACTGACGA
GATCGTCGCCAGAGTGGTCAGCATAATCGACCCCGCCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT192498 WP_000813634.1 NZ_CP069982:54024-54242 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT192498 NZ_CP092437:7952-8215 [Rhizobium sp. K102]
ATGACCAACACAGCAAAGATTCGCCGTCAGGGCGGCGCGACCGTATTTAGCATTCCTCCCGCGCTGTTGAAAATGCTCGG
CGTGGAAGTCGGTGCGGAGTTGACCTTGGTCGTAAGCAATGGAGCGCTTGTCGCAACGCCAACGCGAGCGAAGAAGAGAT
ACACGTTGGCGGAGCTCCTCGAAGGGGCGGATGAGATGTCTGAGCTGAACAAGCAGGCTGCCGCCTGGAACGTCAGCCCG
CCTGTCGGTAATGAAGTATCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References