Detailed information of TA system TA192497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 48779..49380
• Replicon: plasmid unnamed2
• Accession: NZ_CP069982
• Organism: Escherichia coli strain FDAARGOS_1291

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: I6K21_RS24820
• Protein ID: WP_001216034.1
• Coordinates: 48779..49159 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: I6K21_RS24825
• Protein ID: WP_001190712.1
• Coordinates: 49159..49380 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K21_RS24785 (43830)	43830..44762	-	933	WP_023143441.1	homocysteine S-methyltransferase	-
I6K21_RS24790 (44749)	44749..46152	-	1404	WP_001373486.1	S-methylmethionine permease	-
I6K21_RS24795 (46396)	46396..47376	+	981	WP_000019407.1	IS5-like element IS5 family transposase	-
I6K21_RS24800 (47586)	47586..47921	+	336	WP_169329198.1	type I deoxyribonuclease HsdR	-
I6K21_RS24805 (47871)	47871..48284	-	414	Protein_59	integrase core domain-containing protein	-
I6K21_RS24810 (48289)	48289..48567	-	279	Protein_60	pdcB	-
I6K21_RS24815 (48595)	48595..48774	-	180	WP_001513661.1	hypothetical protein	-
I6K21_RS24820 (48779)	48779..49159	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
I6K21_RS24825 (49159)	49159..49380	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6K21_RS24830 (49563)	49563..50495	+	933	Protein_64	type I restriction-modification system subunit M	-
I6K21_RS24835 (50551)	50551..51248	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
I6K21_RS24840 (51385)	51385..52278	-	894	WP_001553853.1	S-4TM family putative pore-forming effector	-
I6K21_RS24845 (52321)	52321..53316	-	996	WP_000246636.1	hypothetical protein	-
I6K21_RS24850 (54024)	54024..54242	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(B) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / dfrA14	-	1..116491	116491
	-	inside	IScluster/Tn	-	-	42873..51248	8375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T192497 WP_001216034.1 NZ_CP069982:c49159-48779 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T192497 NZ_CP092436:c3396601-3396311 [Rhizobium sp. K102]
ATGGGCTTTCGCATTACGGAACAAGCGGAAGAGGACATTGTCGGCATAGCCGAAACGGGCATTCGCCTGTTTGGCTCAGC
GCACGCTCGGCGCTACCATGATGAACTCTACGCCATTTTTGATCTGATTGCTGCCAATCCCCGTATGGCGCGCGAACGGC
ATGAAATTTCTCCTCCGATGCGCATTCATCCGTTCAAGGCCCATCTCGTCGTTTATACGATTGCGGAGGGCGATAATGTT
CTGATTGTCCGTATTCGCCACGGACACGAAGATTGGTCCAGTCAGGTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT192497 WP_001190712.1 NZ_CP069982:c49380-49159 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT192497 NZ_CP092436:c3396855-3396604 [Rhizobium sp. K102]
ATGGCGACCATGAACGTATCGCTGCCCGATCCCATGAAAGATTGGGTGGAAGCGCAAACCAAAACAGGCCGTTATGCCAA
TGCGAGCGATTATGTCCGCGACCTCATCAGGAAAGATCAGGAGCGAAACGACAAGATCGCAGCTATGCAGCGCTATGTCG
ATGAAGGCTTGAAGAGCGGCGTCGGGTCGCGGTCGAAGGACGAGCTCTTTGAGGCGGCGCGGGCGCGCCTGGAAAGCTCT
CGCGGCAACTGA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6